Publications EMF Members

 
2022

Peddie, C.J., Genoud, C., Kreshuk, A. et al. Volume electron microscopyNat Rev Methods Primers 2, 51 (2022)

Munoz O, Banga R, Schelling R, Procopio FA, Mastrangelo A, Nortier P, Ohmiti K, Daraspe J, Cavassini M, Fenwick C, Perez L, Perreau M. Active PD-L1 incorporation within HIV virions functionally impairs T follicular helper cells. PLoS Pathog. 2022 Jul 5;18(7):e1010673. doi: 10.1371/journal.ppat.1010673. Epub ahead of print. PMID: 35788752.

Wai CH, Jin J, Cyrklaff M, Genoud C, Funaya C, Sattler J, Maceski A, Meier S, Heiland S, Lanzer M, Frischknecht F, Kuhle J, Bendszus M, Hoffmann A. Neurofilament light chain plasma levels are associated with area of brain damage in experimental cerebral malaria. Sci Rep. 2022 Jun 24;12(1):10726. doi: 10.1038/s41598-022-14291-x. PMID: 35750882; PMCID: PMC9232608.

McCallin S, Menzi C, Lassen S, Daraspe J, Oechslin F, Moreillon P. Antibiotic Exposure Leads to Reduced Phage Susceptibility in Vancomycin Intermediate Staphylococcus aureus (VISA). Antimicrob Agents Chemother. 2022 Jun 16:e0224721. doi: 10.1128/aac.02247-21. Epub ahead of print. PMID: 35708333.

Santella A, Kolotuev I, Kizilyaprak C, Bao Z. Cross-modality synthesis of EM time series and live fluorescence imaging. Elife. 2022 Jun 6;11:e77918. doi: 10.7554/eLife.77918. PMID: 35666127; PMCID: PMC9213002.

Damien De Bellis, Lothar Kalmbach, Peter Marhavy, Jean Daraspe, Niko Geldner, Marie Barberon. Extracellular vesiculo-tubular structures associated with suberin deposition in plant cell walls.  Nat Commun 13, 1489 (2022). https://doi.org/10.1038/s41467-022-29110-0

Ansermet C, Centeno G, Pradervand S, Harmacek D, Garcia A, Daraspe J, Kocherlakota S, Baes M, Bignon Y, Firsov D. Renal tubular peroxisomes are dispensable for normal kidney function. JCI Insight. 2022 Feb 22;7(4):e155836. doi: 10.1172/jci.insight.155836. PMID: 35191396.

Kian Hématy, Damien De Bellis, Xin Wang, Ari Pekka Mähönen, Niko Geldner. Analysis of exocyst function in endodermis reveals its widespread contribution and specificity of action. Plant Physiology, 2022;, kiac019, https://doi.org/10.1093/plphys/kiac019

Andreas Kolbeck, Peter Marhavý, Damien De Bellis, Baohai Li, Takehiro Kamiya, Toru Fujiwara, Lothar Kalmbach and Niko Geldner. CASP microdomain formation requires cross cell wall stabilization of domains and non-cell autonomous action of LOTR1.eLife 2022;11:e69602 DOI: 10.7554/eLife.69602􏰃

Philippe Glenn, De Bellis Damien, Rose Jocelyn K. C., Nawrath Christiane. Trafficking Processes and Secretion Pathways Underlying the Formation of Plant Cuticles. Frontiers in Plant Science 12, 2022

2021

Romanova DY, Varoqueaux F, Daraspe J, Nikitin MA, Eitel M, Fasshauer D, Moroz LL. Hidden cell diversity in Placozoa: ultrastructural insights from Hoilungia hongkongensis. Cell Tissue Res. 2021 Sep;385(3):623-637. doi: 10.1007/s00441-021-03459-y. Epub 2021 Apr 19. PMID: 33876313; PMCID: PMC8523601.

Vergara HM, Pape C, Meechan KI, Zinchenko V, Genoud C, Wanner AA, Mutemi KN, Titze B, Templin RM, Bertucci PY, Simakov O, Durichen W, Machado P, Savage EL, Schermelleh L, Schwab Y, Friedrich RW, Kreshuk A, Tischer C, Arendt D Cell 2021 Aug 7

Montavon T, Shukeir N, Erikson G, Engist B, Onishi-Seebacher M, Ryan D, Musa Y, Mittler G, Meyer AG, Genoud C, Jenuwein T. Complete loss of H3K9 methylation dissolves mouse heterochromatin organization. Nat Commun. 2021 Jul 16;12(1):4359. doi: 10.1038/s41467-021-24532-8. PMID: 34272378; PMCID: PMC8285382

Franke T, Kolotuev I. Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy. J Vis Exp. 2021 Jul 15;(173). doi: 10.3791/61847. PMID: 34338674.

Rohrbach A, Caron E, Dali R, Brunner M, Pasquettaz R, Kolotuev I, Santoni F, Thorens B, Langlet F. Ablation of glucokinase-expressing tanycytes impacts energy balance and increases adiposity in mice. Mol Metab. 2021 Jul 26:101311. doi: 10.1016/j.molmet.2021.101311. Epub ahead of print. PMID: 34325016

Romanova DY, Varoqueaux F, Daraspe J, Nikitin MA, Eitel M, Fasshauer D, Moroz LL. Hidden cell diversity in Placozoa: ultrastructural insights from Hoilungia hongkongensis. Cell Tissue Res. 2021 Apr 19. doi: 10.1007/s00441-021-03459-y. Epub ahead of print. PMID: 33876313

Carolina Elejalde-Palmett, Ignacio Martinez San Segundo, Imène Garroum, Laurence Charrier, Damien De Bellis, Antonio Mucciolo, Aurore Guerault, Jie Liu, Viktoria Zeisler-Dieh, Asaph Aharoni, Lukas Schreiber, Bénédicte Bakan, Mads H. Clausen, Markus Geisler, Christiane Nawrath. ABCG transporters export cutin precursors for the formation of the plant cuticle. Current Biology, March 2021 https://www.sciencedirect.com/science/article/pii/S0960982221003006?dgcid=coauthor 

Robertas Ursache, Cristovāo De Jesus Vieira Teixeira, Valérie Dénervaud Tendon, Kay Gully, Damien De Bellis, Emanuel Schmid-Siegert, Tonni Grube Andersen, Vinay Shekhar, Sandra Calderon, Sylvain Pradervand, Christiane Nawrath, Niko Geldner & Joop E. M. Vermeer.
GDSL-domain proteins have key roles in suberin polymerization and degradation. Nature Plants, 08 March 2021. https://doi.org/10.1038/s41477-021-00862-9

Kato M, Kolotuev I, Cunha A, Gharib S, Sternberg PW. Extrasynaptic acetylcholine signaling through a muscarinic receptor regulates cell migration. Proc Natl Acad Sci U S A. 2021 Jan 5;118(1):e1904338118. doi: 10.1073/pnas.1904338118. PMID: 33361149; PMCID: PMC7817160.

2020

Nga N T Nguyen, Joaquin Clua, Pallavi V Vetal, Dominique Jacques Vuarambon, Damien de Bellis, Marjorie Pervent, Marc Lepetit, Michael Udvardi, Alexander J Valentine, Yves Poirier.
PHO1 family members transport phosphate from infected nodule cells to bacteroids in Medicago truncatula. Plant Physiology, 25 November 2020
https://academic.oup.com/plphys/advance-article/doi/10.1093/plphys/kiaa016/6003068

Stephanie K Cohen, Marie-Stéphanie Aschtgen, Jonathan B Lynch, Sabrina Koehler, Fangmin Chen, Stéphane Escrig, Jean Daraspe, Edward G Ruby, Anders Meibom, Margaret McFall-Ngai.
Tracking the cargo of extracellular symbionts into host tissues with correlated electron microscopy and nanoscale secondary ion mass spectrometry imaging. Cellular Microbiology 2020 Apr;22(4):e13177
DOI: 10.1111/cmi.13177

Nelson Rojas-Murcia, Kian Hématy, Yuree Lee, Aurélia Emonet, Robertas Ursache, Satoshi Fujita, Damien De Bellis, and Niko Geldner.
High-order mutants reveal an essential requirement for peroxidases but not laccases in Casparian strip lignification. PNAS first published November 2, 2020.
https://doi.org/10.1073/pnas.2012728117

Asier Ullate-Agote, Ingrid Burgelin, Adrien Debry, Carine Langrez, Florent Montange, Rodrigue Peraldi, Jean Daraspe, Henrik Kaessmann, Michel C. Milinkovitch, and Athanasia C. Tzika. Genome mapping of a LYST mutation in corn snakes indicates that vertebrate chromatophore vesicles are lysosome-related organelles. PNAS October 20, 2020 117 (42) 26307-26317.
https://doi.org/10.1073/pnas.2003724117

Mikhail Nikolaev, Olga Mitrofanova, Nicolas Broguiere, Sara Geraldo, Devanjali Dutta, Yoji Tabata, Bilge Elci, Nathalie Brandenberg, Irina Kolotuev, Nikolce Gjorevski, Hans Clevers, Matthias P Lutolf. Homeostatic mini-intestines through scaffold-guided organoid morphogenesis. Nature. 2020 Sep 16. PMID: 32939089
DOI: 10.1038/s41586-020-2724-8

Pasquettaz R, Kolotuev I, Rohrbach A, Gouelle C, Pellerin L, Langlet F. Peculiar protrusions along tanycyte processes face diverse neural and nonneural cell types in the hypothalamic parenchyma. J Comp Neurol. 2020 Jun 8. PMID: 32515035
doi: 10.1002/cne.24965

G. Lê‐Bury, C. Deschamps, C. Kizilyaprak, W. Blanchard, J. Daraspe, A. Dumas, M.A. Gordon, J.C.D. Hinton, B.M. Humbel, F. Niedergang. Increased intracellular survival of Salmonella Typhimurium ST313 in HIV‐1‐infected primary human macrophages is not associated with Salmonella hijacking the HIV compartment. Biology of the Cell, Volume 112, Issue 3, Pages 92-101. March 2020
https://doi.org/10.1111/boc.201900055

Satoshi Fujita, Damien De Bellis, Kai H Edel, Philipp Köster, Tonni Grube Andersen, Emanuel Schmid‐Siegert, Valérie Dénervaud Tendon, Alexandre Pfister, Peter Marhavý, Robertas Ursache, Verónica G Doblas, Marie Barberon, Jean Daraspe, Audrey Creff, Gwyneth Ingram, Jörg Kudla, Niko Geldner. SCHENGEN receptor module drives localized ROS production and lignification in plant roots. The EMBO Journal (2020)39: e103894
https://doi.org/10.15252/embj.2019103894

 
2019

Mumbauer S, Pascual J, Kolotuev I, Hamaratoglu F. Ferritin heavy chain protects the developing wing from reactive oxygen species and ferroptosis. PLoS Genet. 2019 Sep 30;15(9):e1008396.
doi: 10.1371/journal.pgen.1008396

Anna Keppner, Darko Maric, Chloé Sergi, Camille Ansermet, Damien De Bellis, Denise V. Kratschmar, Jérémie Canonica, Petra Klusonova, Robert A. Fenton, Alex Odermatt, Gilles Crambert, David Hoogewijs & Edith Hummler. Deletion of the serine protease CAP2/Tmprss4 leads to dysregulated renal water handling upon dietary potassium depletion. Scientific Reports volume 9, Article number: 19540 (2019)
https://doi.org/10.1038/s41598-019-55995-x

Narasimha S, Nagornov KO, Menin L, Mucciolo A, Rohwedder A, Humbel BM, Stevens M, Thum AS, Tsybin YO, Vijendravarma RK. Drosophila melanogaster cloak their eggs with pheromones, which prevents cannibalism. PLoS Biol. 2019 Jan 10;17(1):e2006012
https://doi.org/10.1371/journal.pbio.2006012

Kolotuev I, Micheva KD, Can Correlative Microscopy Ever Be Easy? An Array Tomography Viewpoint – Correlative Imaging – Wiley Online Library

Caroline Kizilyaprak, Damien De Bellis, Willy Blanchard, Jean Daraspe, Bruno M. Humbel. FIB-SEM Tomography of Biological Samples: Explore the Life in 3D. Chapter 26 in the following book: Biological Field Emission Scanning Electron Microscopy, I. Book Author(s): Roland A. Fleck, Bruno M. Humbel. 15 February 2019
https://doi.org/10.1002/9781118663233.ch26

 

Low IIC, Williams CR, Chong MK, McLachlan IG, Wierbowski BM, Kolotuev I, Heiman MG. Morphogenesis of neurons and glia within an epithelium.
Development, 2019 Feb 20;146(4). DOI:10.1242/dev.171124
https://doi.org/10.1242/dev.171124

Alice Berhin, Damien De Bellis, Rochus B. Franke, Rafael A. Buono, Moritz K. Nowack, and Christiane Nawrath. The Root Cap Cuticle: A Cell Wall Structure for Seedling Establishment and Lateral Root Formation. Cell, 14 February 2019, 176, 1–12. 1
 

2018

Lee KY, Green RA, Gutierrez E, Gomez-Cavazos JS, Kolotuev I, Wang S, Desai A, Groisman A, Oegema K. CYK-4 functions independently of its centralspindlin partner ZEN-4 to cellularize oocytes in germline syncytia. Elife. 2018 Jul 10;7
https://doi.org/10.7554/eLife.36919

Kizilyaprak C, Stierhof YD, Humbel BM. Volume microscopy in biology: FIB-SEM tomography. Tissue Cell. 2018 Sep 26. pii: S0040-8166(18)30262-3.
https://doi.org/10.1016/j.tice.2018.09.006

Eitel M, Francis WR, Varoqueaux F, Daraspe J, Osigus HJ, Krebs S, Vargas S, Blum H, Williams GA, Schierwater B, Wörheide G. Comparative genomics and the nature of placozoan species. PLoS Biol. 2018 Jul 31;16(7):e2005359
https://doi.org/10.1371/journal.pbio.2005359

Burel A, Lavault MT, Chevalier C, Gnaegi H, Prigent S, Mucciolo A, Dutertre S, Humbel BM, Guillaudeux T, Kolotuev I. 2018. A targeted 3D EM and correlative microscopy method using SEM array tomography. Development. 2018 Jun 21;145(12). pii: dev160879. doi: 10.1242/dev.160879.
https://www.ncbi.nlm.nih.gov/pubmed/29802150

Daniel E, Daudé M, Kolotuev I, Charish K, Auld V, Le Borgne R. 2018. Coordination of Septate Junctions Assembly and Completion of Cytokinesis in Proliferative Epithelial Tissues. Current Biology. 2018 May 7;28(9):1380-1391.e4. doi: 10.1016/j.cub.2018.03.034. Epub 2018 Apr 26.
https://www.ncbi.nlm.nih.gov/pubmed/29706514


2017

Pyakurel A, Balmer D, Saba-El-Leil MK, Kizilyaprak C, Daraspe J, Humbel BM, Voisin L, Le YZ, von Lintig J, Meloched S, Roduit R. 2017. Loss of extracellular signal-regulated kinase 1/2 in the retinal pigment epithelium leads to RPE65 decrease and retinal degeneration. Mol Cell Biol, 37, e00295-17.
http://mcb.asm.org/content/37/24/e00295-17.long

Kalmbach L, Hématy K, De Bellis D, Barberon M, Fujita S, Ursache R, Daraspe J, Geldner N. 2017. Transient cell-specific EXO70A1 activity in the CASP domain and Casparian strip localization. Nature Plants 3, Article number: 17058
doi:10.1038/nplants.2017.58
https://www.nature.com/articles/nplants201758

Hoang TV, Kizilyaprak C, Spehner D, Humbel BM, Schultz P. 2017. Automatic segmentation of high pressure frozen and freeze-substituted mouse retina nuclei from FIB-SEM tomograms. J Struct Biol, 123-134.
http://dx.doi.org/10.1016/j.jsb.2016.10.005

Connecting the Molecular Structure of Cutin to Ultrastructure and Physical Properties of the Cuticle in Petals of Arabidopsis. Cover design : plantphysiol.org. Cover image: Antonio Mucciolo with coloring by Willy BlanchardMazurek S, Garroum I, Daraspe J, De Bellis D, Olsson V, Mucciolo A, Butenko MA, Humbel BM, Nawrath C. 2017. Connecting the molecular structure of cutin to ultrastructure and physical properties of the cuticle in petals of Arabidopsis. Plant Physiol, 173, 1146-1163.
https://dx.doi.org/10.1104/pp.16.01637

 

Meier AF, Suter M, Schraner EM, Humbel BM, Tobler K, Ackermann M, Laimbacher AS. 2017. Transfer of anti-rotavirus antibodies during pregnancy and in milk following maternal vaccination with an HSV-1 amplicon vector. Int J Mol Sci,18, 431-450.
http://www.mdpi.com/1422-0067/18/2/431


2016

Fabre G, Garroum I, Mazurek S, Daraspe J, Mucciolo A, Sankar M, Humbel B, Nawrath C. 2016. The ABCG transporter PEC1/ABCG32 is required for the formation of the developing leaf cuticle in Arabidopsis. New Phytol, 209, 192-201.
https://dx.doi.org/10.1111/nph.13608

Garroum I, Bidzinski P, Daraspe J, Mucciolo A, Humbel BM, Morel J-B, Nawrath C. 2016. Cuticular defects in Oryza sativa ATP-binding cassette transporter G31 mutant plants cause dwarfism, elevated defense responses and pathogen resistance Plant & Cell Physiology
https://doi.org/10.1093/pcp/pcw066

Barberon M, Vermeer JEM, De Bellis D, Wang P, Naseer S, Andersen TG, Humbel BM, Nawrath C, Takano J, Salt DE, Geldner N. 2016. Adaptation of Root Function by Nutrient-Induced Plasticity of Endodermal Differentiation. Cell, 164, 1-13.
http://dx.doi.org/10.1016/j.cell.2015.12.021


2015

Loussert Fonta C, Humbel BM. 2015. Correlative Microscopy. Arch Biochem Biophys, 581, 98-110.
https://dx.doi.org/10.1016/j.abb.2015.05.017

Kopp C, Domart-Coulon I, Escrig S, Humbel BM, Hignette M, Meibom A. 2015. Subcellular investigation of photosynthesis-driven carbon assimilation in the symbiotic reef coral Pocillopora damicornis. MBio, 6, 1-9.
http://mbio.asm.org/content/6/1/e02299-14

Kizilyaprak C, Daraspe J, Longo G, Humbel BM. 2015. Investigation of resins suitable for the preparation of biological sample for 3-D electron microscopy. J Struct Biol. 189, 135-146
http://dx.doi.org/10.1016/j.jsb.2014.10.009

Loussert Fonta C,  Leis A, Mathisen C, Bouvier DS, Blanchard W, Volterra A, Lich B, Humbel BM. 2015. Analysis of acute brain slices by electron microscopy: A correlative light–electron microscopy workflow based on Tokuyasu cryo-sectioning. J Struct Biol, 189, 53-61
http://dx.doi.org/10.1016/j.jsb.2014.10.011

Takado Y, Knott G, Humbel BM, Escrig S, Mojgan M, Meibom A., Comment A. 2015. Imaging liver and brain glycogen metabolism at the nanometer scale. Nanomedicine: NBM. 11, 239-245
https://doi.org/10.1016/j.nano.2014.09.007

Takado Y, Knott G, Humbel BM, Masoodi M, Escrig S, Meibom A, Comment A. 2015. Imaging the time-integrated cerebral metabolic activity with subcellular resolution through nanometer-scale detection of biosynthetic products deriving from (13)C-glucose. J Chem Neuroanat, 69, 7-12.
https://doi.org/10.1016/j.jchemneu.2015.09.003


2014

Kizilyaprak C, Daraspe J, Humbel BM. 2014. Focused ion beam scanning electron microscopy in biology. Journal of Microscopy, 254, 109-114.
https://doi.org/10.1111/jmi.12127

Kizilyaprak C, Bittermann AG, Daraspe J, Humbel BM. 2014. FIB-SEM tomography in biology. Methods Mol Biol, 1117, 541-558.
http://link.springer.com/protocol/10.1007%2F978-1-62703-776-1_24

Schwarz H, Humbel BM. 2014. Correlative light and electron microscopy using immunolabeled resin sections. Methods Mol Biol, 1117, 559-592.
http://link.springer.com/protocol/10.1007%2F978-1-62703-776-1_25


2013

Oechslin F, Daraspe J, Giddey M, Moreillon P, Resch G. 2013. In vitro characterization of PlySK1249, a novel phage lysin, and assessment of its antibacterial activity in a mouse model of Streptococcus agalactiae bacteremia. Antimicrob Agents Chemother, 57, 6276-6283.
http://dx.doi.org/10.1128/AAC.01701-13

Broskey NT, Daraspe J, Humbel BM, Amati F. 2013. Skeletal muscle mitochondrial and lipid droplet content assessed with standardJized grid sizes for stereology, J Appl Physiol, 115, 765-770.
http://jap.physiology.org/content/115/5/765.long

Mazurek S, Mucciolo A, Humbel BM, Nawrath C. 2013. Transmission Fourier Transform Infrared Microspectroscopy allows the simultaneous assessment of cutin and cell wall polysaccharides of Arabidopsis petals. Plant J, 74, 880-891.
http://onlinelibrary.wiley.com/doi/10.1111/tpj.12164/

Humbel BM. 2013. Electron microscopy: Cryo-preparation in Roberts GCK (ed), Encyclopedia of Biophysics, Springer-Verlag, 596-600.

Humbel BM. 2013. Electron Microscopy: Classical Sample Preparation in Roberts GCK (ed), Encyclopedia of Biophysics, Springer-Verlag, 646-648.


2012

Karreman MA, Agronskaia AV, van Donselaar EG, Vocking K, Fereidouni F, Humbel BM, Verrips CT, Verkleij AJ, Gerritsen HC. 2012. Optimizing immuno-labeling for correlative fluorescence and electron microscopy on a single specimen. J Struct Biol, 180, 382-386.
https://doi.org/10.1016/j.jsb.2012.09.002

Loussert C, Forestier C-L, Humbel BM. 2012. Correlative Light and Electron Microscopy in Parasite Research. Mol Biol Cell, 111, 59-73.
https://doi.org/10.1016/B978-0-12-416026-2.00004-2

Van Opstal A, Bijvelt J, van Donselaar E, Humbel BM, Boonstra J. 2012. Inhibition of protein kinase B activity induces cell cycle arrest and apoptosis during early G1 phase in CHO cells. Cell Biol Int, 36, 357-365.
https://doi.org/10.1042/CBI20110092

Lamy CM, Sallin O, Loussert C, Chatton JY. 2012. Sodium sensing in neurons with a dendrimer-based nanoprobe. ACS Nano, 6, 1176–1187.
http://pubs.acs.org/doi/abs/10.1021/nn203822t


2011

Forestier C-L, Machu C, Loussert C, Pescher P, Späth GF. 2011. Imaging host cell-Leishmania interaction dynamics implicates parasite motility, lysosome recruitment, and host cell wounding in the infection process. Cell Host Microbe, 9, 319-330.
https://doi.org/10.1016/j.chom.2011.03.011


2010

Nadra K, Quignodon L, Sardella C, Joye E, Mucciolo A, Chrast R, Desvergne B. 2010. PPARγ in placental angiogenesis. Endocrinology, 151, 4969-4981.
https://doi.org/10.1210/en.2010-0131

Hayles MF, de Winter DAM, Schneijdenberg CTWM, Meeldijk JD, Luecken U, Persoon H, de Water J, de Jong F, Humbel BM, Verkleij AJ. 2010. The making of frozen-hydrated, vitreous lamellas from cells for cryo-electron microscopy. J Struct Biol, 172, 180-190.
https://doi.org/10.1016/j.jsb.2010.07.004

Van Laake LW, van Donselaar EG, Monshouwer-Kloots J, Schreurs C, Passier R, Humbel BM, Doevendans PA, Sonnenberg A, Verkleij AJ, Mummery CL. 2010. Extracellular matrix formation after transplantation of human embryonic stem cell-derived cardiomyocytes. Cell Mol Life Sci, 67, 277-290.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2801836/?tool=pubmed

Fontaine T, Beauvais A, Loussert C, Thevenard B, Fulgsang CC, Ohno N, Clavaud C, Prevost MC, Latge J-P. 2010. Cell wall α-1-3-glucans induce the aggregation of germinating connidia of Aspergillus fumigatus. Fungal Genet Biol, 48, 707-712.
https://doi.org/10.1016/j.fgb.2010.04.006