The Leica MP TCS SP8 DIVE upright system is equipped with a multi-photon Insight X3 laser from Spectra Physics that is tunable from 680 to 1300 nm. Four tunes detectors offer flexible detection to the full emission and allow for the separation of strongly overlapping spectra. The incubator box enables 3D time lapse over hours. Next to the scope a fully equipped surgical bench facilitates sample preparations.