Research Projects

 

Aim of our research

Cells live in changing environments. They have evolved complex signal transduction cascades to respond to these changes and mount an appropriate cellular response. Mitogen activated protein kinase (MAPK) are conserved signal transduction modules shared among all eukaryotes. Budding yeast, Saccharomyces cerevisiae, has been a long standing model system to study the regulation of these pathways. Single cell quantitative measurements techniques are needed to fully understand the dynamics of activation of these pathways. Microscopy, combined with microfluidic devices, is ideally suited to generate such data sets. As an example, the relocation of the MAPK Hog1 upon hyper-osmotic stress can be used to quantify signaling activity in this pathway. This type of approaches will be used to study the yeast MAPK pathways. This project relies on the development of new assays based on fluorescent reporters to follow in real time the dynamics and spatial organization of events initiated by the activation of a signal transduction cascade. These measurements will allow to uncover hidden regulatory mechanisms in fundamental signaling pathways conserved in all eukaryotic cells.

Hog1 nuclear relocation in microfluidic device: Fluorescently-tagged Hog1 (green) shuttles to the nucleus of the cell (marked in red) when switch from a normal medium (blue fluorescent dye) to high osmolarity medium.