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The Pelet Lab uses microscopy techniques to study the behavior of single cells. Isogenic cells subjected to the same stimulus can display striking differences in the activation of signaling proteins or in the expression of down-stream genes. We use these differences to understand the underlying mechanisms regulating these signal transduction pathways.

In this video, yeast cells were stressed with 0.2M NaCl. These cells have been tagged with fluorescent proteins. The histones were tagged with a CFP (blue) to localize the nuclei of the cell. The MAPK Hog1 is tagged with mCherry (red) to observe the activation of the signaling cascade. The cells also bear a fluorescent expression reporter (green) which is not expressed in vegetative cells but is strongly induced upon osmotic stress. The quantification of this type of time lapse movies allows to correlate the activity of various elements of a signal transduction cascade in individual cells.

Research Projects

  • Develop new microscopy assays for MAP kinase activity.
  • Understand the regulation of gene expression induced by MAPK pathways.
  • Study the crosstalk between between individual pathways in the yeast MAPK network.

NEWS

15.09.2016 Paper from Victoria Wosika on families on Single Integration Vectors and Gene Tagging plasmids published in Molecular Genetics and Genomics

1.9.2016 Clémence Varidel joins the lab as technician

14.06.2016 Collaborative work with Jan van der Meer group on horizontal gene transfer in bacteria published in PNAS

21.04.2016 Paper from Delphine Aymoz on dynamic protein expression reporters published in Nature Communications

15.02.2016 Posters from all lab members and oral presentation by Delphine Aymoz at the LS2 conference in Lausanne

23.11.2015 Collaborative work lead by Mathias Peter on polarity site establishment is published in Developmental Cell

01.08.2015 Kinase sensors paper from Eric Durandau published in BMC Biology

 

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