University of Lausanne

Protocols

Sample Preparation for Cytometer usage

This document summarizes some information about sample preparation for analyzing and cell sorting and provides additional information about necessary controls, cell concentration and sorting speeds. Please read this if you haven’t used the cell sorting service of the FCF before !

General Sample Preparation

Solid Tissues Processing for Cytometer usage

If you have troubles with sample preparation from solid tissues, this paper could provide insightful details on how to improve your extraction.

Sample Preparation from Solid Tissues

The Flow Cytometry Facility at the University of Lausanne (FCF@UNIL) was created in January 2011. We are currently equipped with 15 analytical flow cytometers and 5 cell sorters located on 2 sites (Biopole SE-D in Epalinges at the CLE campus and in the Agora Cancer Center at the CHUV Bugnon campus).

The FCF offers cell sorting services, technical advice and flow cytometry analysers (both classical and imaging) for use by UNIL/CHUV internal research groups, external academic research groups and companies.

For further information about facility resources and services or how to access them please refer to the specific sections of this website or contact the FCF staff.