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Preprints
2025
- Liu H.W.°, Roisné-Hamelin F.°, Taschner M.°, Collier J., Srinivasan M., Gruber S.*, 2025.
The SMC hinge is a selective gate for obstacle bypass.
Nature Communications
2025.16.10457 [Pubmed]
preprint, 2025 [BioRxiv]
- Roisné-Hamelin F., Liu H.W., Maréchal N., Uchikawa, E., Durand A., Gruber S.*, 2025.
Mechanism of DNA entrapment by a loop-extruding Wadjet SMC motor.
Molecular Cell
2025.09.015 [Pubmed] [Preview by Marc Bramkamp]
- Li Y., Adams D.W., Liu H.W., Shaw S.J., Uchikawa E., Jaskólska M., Stutzmann S., Righi L., Szczelkun M.D., Blokesch M., Gruber S.*, 2025.
Structure and activation mechanism of a Lamassu phage and plasmid defense system.
Nature Structural & Molecular Biology
s41594-025-01677-4 [Pubmed] [Author PDF][Free PDF view]
preprint, 2025 [BioRxiv]
- Houmel M., Pellaton N., Anchimiuk A., Gruber S.*, 2025.
Phage-Encoded TelN Inhibits Bacterial Mre11-Rad50 Nuclease to Protect Hairpin Telomeres.
EMBO Journal
025-00593 [Pubmed]
preprint, 2025 [BioRxiv]
- Roisné-Hamelin F., Liu H.W., Gruber S.*, 2025.
Structure of a type II Wadjet SMC complex from Neobacillus vireti.
Structure
2025.06.004 [Pubmed] [Preview by Kevin Corbett & Amar Deep]
preprint, 2025 [BioRxiv]
- Barth R., Davidson I.F., van der Torre J., Taschner M., Gruber S., Peters J.M., Dekker C.*, 2025.
SMC motor proteins extrude DNA asymmetrically and contain a direction switch.
Cell
2024.12.020 [Pubmed]
preprint, 2023 [BioRxiv]
- Antar H.°, Carraro N.°, Budny H., Gruber S.*, van der Meer J.R.*, 2025.
Orchestrated long-distance gene activation by a ParB-like BisD-CTP DNA clamp in low-frequency transfer competence development in Pseudomonas putida.
Nucleic Acids Research
Vol 53, Issue 15, gkaf802 [Pubmed]
preprint, 2024 [BioRxiv]
- Liu H.W., Gruber S.*, 2025.
DNA Bioconjugation with Polymer Beads Using SNAP-tag® Technology.
Methods in Molecular Biology
Vol 2991, p. 95-108 [Pubmed] [PDF].
2024
- Roisné-Hamelin F.°, Liu H. W.°, Taschner M., Li Y., Gruber S.*, 2024.
Structural basis for plasmid restriction by SMC JET nuclease.
Molecular Cell
Vol 84, Issue 5 [Pubmed]
preprint, 2023 [BioRxiv]
- Taschner M.*, Dickinson J.B., Roisné-Hamelin F., Gruber S.*, 2024.
4G cloning: rapid gene assembly for expression of multisubunit protein complexes in diverse hosts.
Life Science Alliance
vol. 8 no. 1 e202402899 [Pubmed]
preprint, 2024 [BioRxiv]
- Tišma M., Bock F.P., Kerssemakkers J., Antar H., Japaridze A., Gruber S., Dekker C.*, 2024.
Direct observation of a crescent-shape chromosome in expanded Bacillus subtilis cells.
Nature Communications
Vol 15, 2737 [Pubmed]
preprint, 2023 [BioRxiv]
- Liu X., Van Maele L., Matarazzo L., Soulard D., Duarte da Silva V.A., de Bakker V., Dénéréaz J., Bock F.P., Taschner M., Ou J., Gruber S., Nizet V., Sirard J.-C.*, Veening J.-W.*, 2024.
A conserved antigen induces respiratory Th17-mediated serotype-independent protection against pneumococcal superinfection.
Cell Host & Microbe
Vol 32, 1-11 [Pubmed]
preprint, 2023 [BioRxiv]
- Tišma M., Kaljevic J., Gruber S., Le T.B.K., Dekker C.*, 2024.
Connecting the dots: key insights on ParB for chromosome segregation from single-molecule studies.
FEMS Microbiol Rev
Vol 48 (1) [Pubmed]
- Gonzalez A.M., Tisma M., Analikwu B.T., Barth A., Janissen R., Antar H., Kemps G., Gruber S., Dekker C.*, 2024.
DNA supercoiling enhances DNA condensation by ParB proteins.
Nucleic Acids Research
gkae936 [Pubmed]
preprint, 2024 [BioRxiv]
2023
- Taschner M., Gruber S.*, 2023.
DNA segment capture by Smc5/6 holo-complexes.
Nature Structural & Molecular Biology
10.1038/s41594-023-00956-2 [Pubmed]
preprint, 2022 [BioRxiv]
- Liu H.W., Roisné-Hamelin F., Gruber S.*, 2023.
SMC-based immunity against extrachromosomal DNA elements.
Biochemical Society Transactions
BST20221395 [Pubmed]
- Antar H., Gruber S.*, 2023.
VirB, a transcriptional activator of virulence in Shigella flexneri, uses CTP as a cofactor.
Communications Biology
6:1024 [Pubmed]
preprint, 2023 [BioRxiv]
- Tišma M., Janissen R., Antar H., Martin Gonzalez A., Barth R., Beekman T., van der Torre J., Michieletto D., Gruber S., Dekker C.*, 2023.
Dynamic ParB-DNA interactions initiate and maintain a partition condensate for bacterial chromosome segregation.
Nucleic Acids Research
gkad868 [Pubmed]
preprint, 2023 [BioRxiv]
2022
- Liu H.W.°, Roisné-Hamelin F.°, Beckert B., Li Y., Myasnikov A., Gruber S.*, 2022.
DNA-measuring Wadjet SMC ATPases restrict smaller circular plasmids by DNA cleavage.
Molecular Cell
Vol 82, Issue 24 [Pubmed]
preprint, 2022 [BioRxiv]
- Roberts D.M., Anchimiuk A., Kloosterman T.G., Murray H., Wu L.J, Gruber S., Errington J.*, 2022.
Chromosome remodelling by SMC/Condensin in B. subtilis is regulated by Soj/ParA during growth and sporulation.
PNAS
Vol 40, Issue 9 [Pubmed]
preprint, 2021 [BioRxiv]
- Bock F.P., Liu H.W., Anchimiuk A., Diebold-Durand M.-L., Gruber S.*, 2022.
A joint-ParB interface promotes Smc DNA recruitment.
Cell Reports
Vol 119, Issue 41 [Pubmed]
preprint, 2021 [BioRxiv]
- Tisma M., Panoukidou M., Antar H., Soh Y.-M., Barth R., Pradhan B., van der Torre J., Michieletto D., Gruber S., Dekker C.*, 2022.
ParB proteins can bypass DNA-bound roadblocks by dimer-dimer recruitment.
Science Advances
Vol 8, Issue 26 [Pubmed]
preprint, 2021 [BioRxiv]
- Nomidis S.S., Carlon E., Gruber S., Marko J. F.*, 2022.
DNA tension-modulated translocation and loop extrusion by SMC complexes revealed by molecular dynamics simulations.
Nucleic acids research
gkac268 [Pubmed]
preprint, 2021 [BioRxiv]
2021
- Antar H.°, Soh Y.-M.°, Zamuner S., Bock F. P., Anchimiuk A., De Los Rios P., Gruber S.*, 2021.
Relief of ParB autoinhibition by parS DNA catalysis and ParB recycling by CTP hydrolysis promote bacterial centromere assembly.
Science Advances
Vol 7, Issue 41 [Pubmed]
preprint, 2021 [BioRxiv]
- Taschner M., Basquin J., Steigenberger B., Schaefer I., Soh Y.-M., Basquin C., Lorentzen E., Räschle M., Scheltema R. A., Gruber S.*, 2021.
Nse5/6 inhibits the Smc5/6 ATPase and modulates DNA substrate binding.
EMBO Journal
e107807 [Pubmed]
preprint, 2021 [BioRxiv]
- Anchimiuk A., Lioy V. S., Minnen A., Boccard F., Gruber S.*, 2021.
A low Smc flux avoids collisions and facilitates chromosome organization in B. subtilis.
eLife
65467 [Pubmed]
preprint, 2020 [BioRxiv]
- Gallay C.°, Sanselicio S.°, Anderson M. E., Soh Y.-M., Liu X., Stamsas G. A., Pelliciari S., van Raaphorst R., Dénéréaz J., Kjos M., Murray H., Gruber S., Grossman A. D., Veening J.-W.*, 2021.
CcrZ is a pneumococcal spatiotemporal cell cycle regulator that interacts with FtsZ and controls DNA replication.
Nature Microbiology
021-00949-1 [Pubmed]
preprint, 2019 [BioRxiv]
- Vazquez Nunez R. J.°, Polyhach Y.°, Soh Y.-M., Jeschke G., Gruber S.*, 2021.
Gradual opening of Smc arms in prokaryotic condensin.
Cell Reports
35(4) 109051 [Pubmed]
preprint, 2021 [BioRxiv]
2020
- Soh Y.-M., Basquin J., Gruber S.*, 2020.
A rod conformation of the Pyrococcus furiosus Rad50 coiled coil.
Proteins
prot.26005 [Pubmed] [PDB: 6ZFF]
preprint, 2020 [BioRxiv]
- Metwaly G., Wu Y., Peplowska K., Röhrl J., Soh Y.-M., Bürmann F., Gruber S., Storchova Z.*, 2020.
Phospho-regulation of the Shugoshin-Condensin interaction at the centromere in budding yeast.
PLOS Genetics
16(8) [Pubmed]
preprint, 2019 [BioRxiv]
- Jeon J.-H., Lee H.-S., Shin H.-C., Kwak M.-J., Kim Y.-G., Gruber S. and Oh B.-H.*, 2020.
Evidence for binary Smc complexes lacking kite subunits in archaea.
IUCrJ
7(2) [Pubmed]
- Prassler J., Simon F., Ecke M., Gruber S. and Gerisch G.* 2020.
Decision making in phagocytosis.
AEMB
1246:71-81 [Pubmed] [PDF]
2019
- Soh Y.-M., Davidson I. F., Zamuner S., Basquin J., Taschner M., Bock F. P., Veening J.-W., De Los Rios P., Peters J.-M., Gruber S.*, 2019.
Self-organization of parS centromeres by the ParB CTP hydrolase.
Science
360(6469) p. 1129-1133 [Pubmed] [Free] [PDB: 6SDK] [PDF] [F1000_recommendations] [Perspective by Barbara Funnell]
- Vazquez Nunez R.°, Ruiz Avila L. B.°, Gruber S.*, 2019.
Transient DNA occupancy of the SMC interarm space in prokaryotic condensin.
Molecular Cell
75(5) p. 1-15 [Pubmed] [Free full-text] [Preview by Tomoko Nishiyama]
preprint, 2018 [BioRxiv]
- Marko J. F.*, De Los Rios P., Barducci A., Gruber S., 2019.
DNA-segment-capture model for loop extrusion by structural maintenance of chromosome (SMC) protein complexes.
Nucleic Acids Research
gkz497 [Pubmed]
preprint, 2018 [BioRxiv]
- Diebold-Durand M.-L., Bürmann F., Gruber S.*, 2019.
High-throughput allelic replacement screening in Bacillus subtilis.
Methods in Molecular Biology
2004 p. 49-61 [Pubmed] [PDF]
2018
- Pfeiffer F., Zamora-Lagos M.-A., Blettinger M., Yeroslavic A., Dahl A., Gruber S.*, Habermann B. H.*, 2018.
The complete and fully assembled genome sequence of Aeromonas salmonicida subsp. pectinolytica and its comparative analysis with other Aeromonas species: investigation of the mobilome in environmental and pathogenic strains.
BMC Genomics
19(1):20 [Pubmed]
- Gruber S., 2018.
SMC complexes sweeping through the chromosome: Going with the flow and against the tide.
Current Opinion in Microbiology
42:96-103 [Pubmed] [PDF]
- Stockmar I., Feddersen H., Cramer K., Gruber S., Jung K., Bramkamp M.*, Shin J. Y.* 2018.
Optimization of sample preparation and green color imaging using the mNeonGreen fluorescent protein in bacterial cells for photoactivated localization microscopy.
Scientific Reports
8(1): 10137 [Pubmed]
2017
- Diebold-Durand M.-L.°, Lee H.°, Ruiz Avila L.°, Noh H., Shin H.-C., Im H., Bock F. P., Bürmann F., Durand A., Basfeld A., Ham S., Basquin J., Oh B.-H.*, Gruber S.*, 2017.
Structure of full-length SMC and rearrangements required for chromosome organization.
Molecular Cell
67(2) p. 334-347 [Web of Science] [Pubmed] [PDB: 5NMO, 5NNV]
- Bürmann F., Basfeld A., Vazquez Nunez R., Diebold-Durand M.-L., Wilhelm L., Gruber S.*, 2017.
Tuned SMC arms drive chromosomal loading of prokaryotic condensin.
Molecular Cell
65(5) p. 861-872 [Web of Science] [Pubmed] [F1000 recommendation]
- Gruber S., 2017.
Shaping Chromosomes by DNA Capture and Release: Gating the SMC Rings.
Current Opinion in Cell Biology
46:87-93 [Web of Science] [Pubmed] [PDF]
- Wilhelm L., Gruber S.*, 2017.
A Chromosome Co-Entrapment Assay to Study Topological Protein–DNA Interactions.
Methods in Molecular Biology
1624 p. 117-126 [Web of Science] [Pubmed] [PDF]
An updated version of the protocol (using agarose microbeads instead of agarose plugs) is available here: [DOI].
2016
- Minnen A.°, Bürmann F.°, Wilhelm L., Anchimiuk A., Diebold-Durand M.-L., Gruber S.*, 2016.
Control of Smc Coiled Coil Architecture by the ATPase Heads Facilitates Targeting to Chromosomal ParB/parS and Release onto Flanking DNA.
Cell Reports
14(8) p. 2003-2016 [Web of Science] [Pubmed]
- Haering C. H., Gruber S., 2016.
SnapShot: SMC Protein Complexes Part I.
Cell
164(1-2) p. 326-6.e1 [Web of Science] [Pubmed]
- Haering C. H., Gruber S., 2016.
SnapShot: SMC Protein Complexes Part II.
Cell
164(4) p. 818.e1 [Web of Science] [Pubmed]
- Wilhelm L., Gruber S., 2016.
Chromosom in Schleifen: SMC-Komplexe als molekulare Kabelbinder?
BioSpektrum
22(4) p. 356-358 [PDF]
2015
- Soh Y.-M.°, Bürmann F.°, Shin H. C., Oda T., Jin K. S., Toseland C. P., Kim C., Lee H., Kim S. J., Kong M. S., Durand-Diebold M.-L., Kim Y. G., Kim H. M., Lee N. K., Sato M., Oh B. H.*, Gruber S.*, 2015.
Molecular basis for SMC rod formation and its dissolution upon DNA binding.
Molecular Cell
57(2) p. 290-303 [Web of Science] [Pubmed]
- Wilhelm L., Bürmann F., Minnen A., Shin H. C., Toseland C. P., Oh B.-H., Gruber S.*, 2015.
SMC condensin entraps chromosomal DNA by an ATP hydrolysis dependent loading mechanism in Bacillus subtilis.
eLife
4:e06659 [Web of Science] [Pubmed]
- Palecek J. J.*, Gruber S.*, 2015.
Kite Proteins: a Superfamily of SMC/Kleisin Partners Conserved Across Bacteria, Archaea, and Eukaryotes.
Structure
23(12) p. 2183-2190. [Web of Science] [Pubmed]
- Kang H.A., Shin H.C., Kalantzi A.S., Toseland C.P., Kim H. M., Gruber S., Peraro M. D., Oh B.-H.*, 2015.
Crystal structure of Hop2-Mnd1 and mechanistic insights into its role in meiotic recombination.
Nucleic Acids Research
43(7) p. 3841-3856 [Web of Science] [Pubmed]
- Attaiech L., Minnen A., Kjos M., Gruber S., Veening J.-W.*, 2015.
The ParB-parS Chromosome Segregation System Modulates Competence Development in Streptococcus pneumoniae.
Mbio
6(4) p. e00662 [Web of Science] [Pubmed]
- Bürmann F., Gruber S., 2015.
SMC condensin: promoting cohesion of replicon arms.
Nature Structural & Molecular Biology
22(9) p. 653-655 [Web of Science] [Pubmed] [PDF] [PDB: 3ZGX]
2014
- Gruber S.*, Veening J.-W., Bach J., Blettinger M., Bramkamp M., Errington J.*, 2014.
Interlinked sister chromosomes arise in the absence of condensin during fast replication in B. subtilis.
Current Biology
24(3) p. 293-298 [Web of Science] [Pubmed]
- Gligoris T. G., Scheinost J. C., Bürmann F., Petela N., Chan K. L., Uluocak P., Beckouët F., Gruber S., Nasmyth K.*, Löwe J.*, 2014.
Closing the cohesin ring: structure and function of its Smc3-kleisin interface.
Science
346(6212) p. 963-967 [Web of Science] [Pubmed]
- Gruber S., 2014.
Multilayer chromosome organization through DNA bending, bridging and extrusion.
Current Opinion In Microbiology
22 p. 102-110 [Web of Science] [Pubmed]
2013
2011
2009
2006
- Gruber S., Arumugam P., Katou Y., Kuglitsch D., Helmhart W., Shirahige K., Nasmyth K.*, 2006.
Evidence that loading of cohesin onto chromosomes involves opening of its SMC hinge.
Cell
127(3) p. 523-537 [Web of Science] [Pubmed]
- Arumugam P., Nishino T., Haering C.H., Gruber S., Nasmyth K.*, 2006.
Cohesin’s ATPase activity is stimulated by the C-terminal Winged-Helix domain of its kleisin subunit.
Current Biology
16(20) p. 1998-2008 [Web of Science] [Pubmed]
2004
2003
- Gruber S.°, Haering C. H.°, Nasmyth K.*, 2003.
Chromosomal cohesin forms a ring.
Cell
112(6) p. 765-777 [Web of Science] [Pubmed]
- Arumugam P., Gruber S., Tanaka K., Haering C. H., Mechtler K., Nasmyth K.*, 2003.
ATP hydrolysis is required for cohesin’s association with chromosomes.
Current Biology
13(22) p. 1941-1953 [Web of Science] [Pubmed]
- Buonomo S. B., Rabitsch K. P., Fuchs J., Gruber S., Sullivan M., Uhlmann F., Petronczki M., Tóth A., Nasmyth K.*, 2003.
Division of the nucleolus and its release of CDC14 during anaphase of meiosis I depends on separase, SPO12, and SLK19.
Developmental Cell
4(5) p. 727-739 [Web of Science] [Pubmed]
