Services

Sample
preparation

  • Protein extraction, quantification & digestion
  • Peptide clean-up
  • Phosphopeptide enrichment

Simple Western

Targeted quantitation of proteins using antibodies & automated capillary electrophoresis

Mass
spectrometry

  • Protein identification
  • Protein quantitation by label-free (LFQ) / isobaric (TMT) / metabolic labelling (SILAC)
  • Identification and quantitation of Post Translational Modifications (PTM)
  • Data Dependant or Independant Acquisition workflows (DDA/DIA)

Typical workflows & applications

All workflows encompass data processing, filtering, normalization and statistical testing, accompanied by comprehensive reporting.

Total proteome profiling

Typical output: 6’000-8’000 protein groups identified and quantitated with high data completeness
Amount needed: 20 ug proteins
Format : any number of samples

  • Extraction of proteins from cells or tissue samples
  • Protein digestion and peptide clean-up
  • MS analysis by DIA direct or via project-specific library

Protein interactions

Typical output: 500 – 2’500 protein groups
Amount needed: experiment-dependent
Format : any number of samples

  • Following affinity purification, protein digestion on-beads or after elution.
  • MS analysis by DDA, label-free quantitation.
  • Variants :
    • Dedicated digestion procedures for proximity labelling samples (streptavidin beads).
    • MS analysis on the Orbitrap Fusion with FAIMS interface for increasing depth by ion mobility fractionation of peptides.
    • Classical geLC-MS/MS workflow available on demand.

Phosphoproteomics (Phospho-TMT 16-plex)

Typical output: 10’000-15’000 phosphosites identified and quantitated with data completeness > 80%
Amount needed: 150 ug proteins
Format : Batches of 16 samples to be compared directly

  • Extraction of proteins from cells or tissue samples
  • Protein digestion and peptide clean-up
  • Labelling with TMT Pro reagents
  • Phosphopeptide enrichment by IMAC
  • MS analysis on FAIMS-Orbitrap instrument with fractionation by ion mobility to increase depth
  • Parallel profiling of total proteomes on the same samples

See our technology page for more information on our instrumentation and our data analysis workflows.