{"id":300,"date":"2023-07-11T16:16:48","date_gmt":"2023-07-11T14:16:48","guid":{"rendered":"https:\/\/wp.unil.ch\/paf\/?page_id=300"},"modified":"2025-01-30T16:41:43","modified_gmt":"2025-01-30T15:41:43","slug":"services","status":"publish","type":"page","link":"https:\/\/wp.unil.ch\/paf\/services\/","title":{"rendered":"Services"},"content":{"rendered":"\n
\n
\n

Sample
preparation<\/h2>\n\n\n
\n
\"\"<\/figure>\n<\/div>\n\n\n

Protein extraction, quantification & digestion<\/p>\n\n\n\n

Peptide clean-up<\/p>\n\n\n\n

Phosphopeptide enrichment<\/p>\n<\/div>\n\n\n\n

\n

Simple Western<\/h2>\n\n\n
\n
\"\"<\/figure>\n<\/div>\n\n\n

Targeted quantitation of proteins using antibodies & automated capillary electrophoresis
<\/p>\n<\/div>\n\n\n\n

\n

Mass
spectrometry<\/h2>\n\n\n
\n
\"\"<\/figure>\n<\/div>\n\n\n

Protein identification<\/p>\n\n\n\n

Protein quantitation by label-free (LFQ<\/strong>) \/ isobaric (TMT<\/strong>) \/ metabolic labelling (SILAC)<\/strong><\/p>\n\n\n\n

Identification & quantitation of Post Translational Modifications (PTM<\/strong>)<\/p>\n\n\n\n

Data Dependant or Independant Acquisition workflows (DDA\/DIA<\/strong>)<\/p>\n<\/div>\n<\/div>\n\n\n\n

Typical workflows & applications<\/h2>\n\n\n\n

All workflows encompass data processing, filtering, normalization and statistical testing, accompanied by comprehensive reporting.<\/p>\n\n\n\n

Total proteome profiling<\/h3>\n\n\n\n

Typical output<\/strong>: 6’000-8’000 protein groups identified and quantitated with high data completeness<\/em>
Amount needed<\/strong>: 20 ug proteins<\/em>
Format<\/strong> : any number of samples<\/em><\/p>\n\n\n\n