{"id":1054,"date":"2023-10-06T15:42:40","date_gmt":"2023-10-06T13:42:40","guid":{"rendered":"https:\/\/wp.unil.ch\/paf\/?page_id=1054"},"modified":"2023-10-19T17:11:23","modified_gmt":"2023-10-19T15:11:23","slug":"data-processing","status":"publish","type":"page","link":"https:\/\/wp.unil.ch\/paf\/data-processing\/","title":{"rendered":"Data processing"},"content":{"rendered":"\n<h2 class=\"wp-block-heading\">What you will get<\/h2>\n\n\n\n<p>Depending on analytical workflow, results will be provided in different formats:<\/p>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><td><strong><em>Workflow<\/em><\/strong><\/td><td><strong><em>Results<\/em><\/strong><\/td><td><strong><em>Tutorial<\/em><\/strong><\/td><\/tr><tr><td>Protein identification <br>(by Mascot)<\/td><td>&#8211; Scaffold file (.sf3)<br>&#8211; Excel table<\/td><td><a href=\"https:\/\/wp.unil.ch\/paf\/files\/2023\/10\/Shotgun_data_guide_v5.6-5.pdf\" data-type=\"link\" data-id=\"https:\/\/wp.unil.ch\/paf\/files\/2023\/10\/Shotgun_data_guide_v5.6-5.pdf\">Shotgun analysis<\/a><\/td><\/tr><tr><td>Targeted protein quantification <br>(Simple Western)<\/td><td>&#8211; Excel table <br>&#8211; Report<\/td><td><a href=\"https:\/\/www.bio-techne.com\/pdf-download-arena-document\/brochure\/pl6-0002\" data-type=\"link\" data-id=\"https:\/\/www.bio-techne.com\/pdf-download-arena-document\/brochure\/pl6-0002\">Western capillary<\/a><\/td><\/tr><tr><td>Protein quantification<br>(labeled or label-free)<\/td><td>&#8211; Excel table (with statistics)<br>&#8211; Report<\/td><td><a href=\"https:\/\/wp.unil.ch\/paf\/files\/2023\/07\/PAF_Protein-Quant_tutorial_v4-1.pdf\" data-type=\"link\" data-id=\"https:\/\/wp.unil.ch\/paf\/files\/2023\/07\/PAF_Protein-Quant_tutorial_v4-1.pdf\">Quantitative analysis<\/a><\/td><\/tr><tr><td>Phosphorylation quantification <br>(combined with TMT)<\/td><td>&#8211; Excel table (with statistics)<br>&#8211; Report<\/td><td><a href=\"https:\/\/wp.unil.ch\/paf\/files\/2023\/07\/PAF_Phospho_proteomics_tutorial_v3.pdf\" data-type=\"link\" data-id=\"https:\/\/wp.unil.ch\/paf\/files\/2023\/07\/PAF_Phospho_proteomics_tutorial_v3.pdf\">Phosphoproteomics analysis<\/a><\/td><\/tr><\/tbody><\/table><figcaption class=\"wp-element-caption\">For proteomics quantitative experiments, you receive a <strong>table <\/strong>with a list of proteins (or modification sites in case of PTM\u2019s) and columns with associated <strong>quantitative values<\/strong>. Some <strong>statistical <\/strong>tests to compare conditions are performed and a <strong>basic pathway enrichment<\/strong> analysis is included.<\/figcaption><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\">Publication<\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Specific material and methods for proteomics analyses are available on demand.<br><span style=\"text-decoration: underline\">Sample ID numbers must be provided in your request<\/span>.<\/li>\n\n\n\n<li>Most journals ask for data submission to a repository before article publication. For now, our facility takes care of data submission to <a href=\"https:\/\/www.proteomexchange.org\/\" data-type=\"link\" data-id=\"https:\/\/www.proteomexchange.org\/\">ProteomeXchange<\/a> repository. Please follow the instructions provided in this <a href=\"https:\/\/wp.unil.ch\/paf\/files\/2023\/07\/PRIDE-submission_TEMPLATE_v3.docx\" data-type=\"link\" data-id=\"https:\/\/wp.unil.ch\/paf\/files\/2023\/07\/PRIDE-submission_TEMPLATE_v3.docx\">tutorial<\/a>.<\/li>\n\n\n\n<li>Regarding PAF acknowledgements or eventual co-authorship, please refer to our <a href=\"https:\/\/wp.unil.ch\/paf\/policy\/\" data-type=\"link\" data-id=\"https:\/\/wp.unil.ch\/paf\/policy\/\">policy and publication guidelines<\/a>.<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>What you will get Depending on analytical workflow, results will be provided in different formats: Workflow Results Tutorial Protein identification (by Mascot) &#8211; Scaffold file (.sf3)&#8211; Excel table&hellip;<\/p>\n","protected":false},"author":1002529,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-full-width.php","meta":{"_seopress_robots_primary_cat":"","_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","footnotes":""},"class_list":["post-1054","page","type-page","status-publish","has-post-thumbnail"],"_links":{"self":[{"href":"https:\/\/wp.unil.ch\/paf\/wp-json\/wp\/v2\/pages\/1054","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wp.unil.ch\/paf\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wp.unil.ch\/paf\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wp.unil.ch\/paf\/wp-json\/wp\/v2\/users\/1002529"}],"replies":[{"embeddable":true,"href":"https:\/\/wp.unil.ch\/paf\/wp-json\/wp\/v2\/comments?post=1054"}],"version-history":[{"count":1,"href":"https:\/\/wp.unil.ch\/paf\/wp-json\/wp\/v2\/pages\/1054\/revisions"}],"predecessor-version":[{"id":1528,"href":"https:\/\/wp.unil.ch\/paf\/wp-json\/wp\/v2\/pages\/1054\/revisions\/1528"}],"wp:attachment":[{"href":"https:\/\/wp.unil.ch\/paf\/wp-json\/wp\/v2\/media?parent=1054"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}