{"id":1133,"date":"2015-03-27T11:14:43","date_gmt":"2015-03-27T10:14:43","guid":{"rendered":"http:\/\/wp.unil.ch\/hardtkelab\/?page_id=1133"},"modified":"2022-10-10T08:23:08","modified_gmt":"2022-10-10T06:23:08","slug":"phloem-differentiation-hormones","status":"publish","type":"page","link":"https:\/\/wp.unil.ch\/hardtkelab\/research-projects\/phloem-differentiation-hormones\/","title":{"rendered":"Phloem Differentiation & Hormones"},"content":{"rendered":"

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Vascular differentiation and its relation to plant hormone pathways has become a major focus of our lab over the years. The importance of the vascular system for plant development cannot be overstated.\u00a0Its\u00a0evolution enabled plants to effectively colonize land, which has had a long-lasting impact that shaped earth history and the extant biosphere.<\/em><\/strong> Land plants are autotroph organisms that extract inorganic nutrients from the soil and\u00a0carbon dioxide\u00a0from the atmosphere to build up organic biomass, thereby converting the energy harvested by photosynthesis from sunlight. Nutrients, carbon dioxide,\u00a0light\u00a0and\u00a0water all limit plant productivity, either because they constitute primary substrates for photosynthesis reactions, or because they are needed as co-factors or\u00a0integral components of the photosynthetic machinery. Competition for these resources and their\u00a0fluctuations over geological time has driven plant evolution towards the elaborate shoot and root systems characteristic of the higher plants that dominate the land biosphere today. Their vascular tissues allowed\u00a0this body plan expansion, because they\u00a0permit\u00a0the long distance separation of the location of water and nutrient acquisition from the location of photosynthesis. At the heart of the vasculature, xylem transports water and inorganic nutrients absorbed by the root system to aboveground organs, whereas phloem distributes photosynthetic and other organic metabolites throughout the plant. The two tissues always develop in close proximity to one another, because water exchange between xylem and phloem is necessary to allow bulk transport of phloem sap, even against the water potential gradient. Xylem and phloem\u00a0are continuously formed during organ formation from the stem cell niches in plant meristems, the growth apices of plant body axes. For example, in primary roots of Arabidopsis thaliana<\/em> (Arabidopsis), the vascular tissues are formed from stem cells located at the root tip. Because xylem as well as phloem\u00a0formation can be followed along the spatio-temporal gradient of single cell files by confocal microscopy in the Arabidopsis root, it is a particularly well-suited system to investigate this process. We mostly focus on the development of the phloem sap conduits, which are formed by connected sieve elements. Surprisingly little is known about the molecular-genetic control of sieve element development, a differentiation process that\u00a0encompasses major cellular rearrangments, such as cell elongation, wall thickening and enucleation.\u00a0Pertinent\u00a0publications on this topic include the following:<\/p>\n

Ana Cecilia Aliaga Fandino and Christian S. Hardtke* (2022): Auxin transport in developing protophloem: a case study in canalization.\u00a0<\/strong>Journal of Plant Physiology<\/strong><\/em>, DOI:10.1016\/j.jplph.2021.153594.<\/a><\/p>\n

Moritz Graeff and Christian S. Hardtke (2021): Metaphloem development in the Arabidopsis root tip.<\/strong>Development,<\/strong><\/em> DOI:10.1242\/dev.199766.<\/a><\/p>\n

Samuel W. H. Koh, Petra Marhava, Surbhi Rana, Alina Graf, Bernard Moret, Alkistis E. L. Bassukas, Melina Zourelidou, Martina Kolb, Ulrich Z. Hammes, Claus Schwechheimer\u00a0and Christian S. Hardtke (2021): Mapping and engineering of auxin-induced plasma-membrane-dissociation in BRX family proteins. The Plant Cell<\/em><\/strong>, DOI:10.1093\/plcell\/koab076.<\/a><\/p>\n

Bernard Moret, Petra Marhava, Ana Cecilia Aliaga Fandino, Christian S. Hardtke*\u00a0 and Kirsten ten Tusscher*\u00a0 (2020): Local auxin competition explains fragmented differentiation patterns<\/strong><\/span>.\u00a0<\/span><\/strong>Nature Communications<\/em>,\u00a0<\/span><\/b>doi.org\/10.1038\/s41467-020-16803-7<\/span>.<\/a><\/p>\n

Moritz Graeff\u2020<\/sup>, Surbhi Rana\u2020<\/sup>, Petra Marhava, Bernard Moret and Christian S. Hardtke (2020): Local and systemic effects of brassinosteroid perception in developing phloem. Current Biology<\/em>,\u00a0<\/span><\/b>Vol. 30: pp. 1626-1638<\/span><\/a>.<\/p>\n

Petra Marhava, Ana Cecilia Aliaga Fandino, Samuel W.H. Koh, Adriana Jeli\u00adnkova, Martina Kolb, Dorina P. Janacek, Alice Breda, Pietro Cattaneo, Ulrich Z. Hammes, Jan Petrasek and Christian S. Hardtke (2020):\u00a0Plasma membrane domain patterning and self-reinforcing polarity in Arabidopsis.\u00a0<\/b>Developmental Cell,<\/strong> <\/i><\/span>doi.org\/10.1016\/j.devcel.2019.11.015.<\/a><\/p>\n

Alice S. Breda\u2020<\/sup>, Ora Hazak\u2020<\/sup>, Patrick Schultz\u2020<\/sup>, Pauline Anne, Moritz Graeff, R\u00fcdiger Simon and\u00a0Christian S. Hardtke\u00a0<\/span>(2019): A cellular insulator against CLE45 peptide signaling. Current Biology, <\/span><\/em><\/strong>Vol. 29: pp. 2501-2508.<\/a><\/p>\n

Petra Marhava, A. E. Lanassa Bassukas, Melina Zourelidou, Martina Kolb, Bernard Moret, Astrid Fastner, Waltraud X. Schulze, Pietro Cattaneo, Ulrich Z. Hammes, Claus Schwechheimer*<\/sup>\u00a0and Christian S. Hardtke*<\/sup>\u00a0<\/sup>(2018): A molecular rheostat adjusts auxin flux to promote root protophloem differentiation. Nature, <\/span><\/em><\/strong>Vol. 558: pp. 297-300.<\/a><\/p>\n

Pauline Anne, Amelia Amiguet-Vercher, Benjamin Brandt, Lothar Kalmbach, Niko Geldner, Michael Hothorn\u00a0and Christian S. Hardtke\u00a0<\/sup>(2018): CLERK is a novel receptor kinase required for sensing of root-active CLE peptides in Arabidopsi<\/strong>s. Development, <\/span><\/em><\/strong>Vol. 145: pp. dev.162354.<\/a><\/p>\n

Pauline Anne and\u00a0Christian S. Hardtke\u00a0<\/sup>(2018):\u00a0Phloem function and developmen \u2014\u00a0biophysics meets genetics. Current Opinion in Plant Biology, <\/span><\/em><\/strong>Vol. 43: pp. 22-28.<\/a><\/p>\n

Alice S. Breda, Ora Hazak\u00a0and Christian S. Hardtke\u00a0(2017): Phosphosite charge rather than shootward localization determines OCTOPUS activity in root protophloem<\/strong>. Proceedings of<\/em> the National Academy of Sciences U.S.A.<\/em><\/span><\/strong>,<\/span>\u00a0Vol. 114: pp.\u00a0E5721<\/span>–<\/span>E5730.<\/span><\/a><\/p>\n

Ora Hazak\u2020<\/sup>, Benjamin Brandt\u2020<\/sup>, Pietro Cattaneo, Julia Santiago, Antia Rodriguez-Villalon, Michael Hothorn*<\/sup>\u00a0and Christian S. Hardtke*<\/sup>\u00a0(2017): Perception of root-active CLE peptides requires CORYNE function in the phloem vasculature<\/strong>. EMBO Reports<\/em><\/span><\/strong>,<\/span> Vol. 18: pp. 1367-1381.<\/a><\/p>\n

Yeon Hee Kang, Alice S. Breda and Christian S. Hardtke (2017):\u00a0Brassinosteroid signaling directs formative cell divisions and protophloem differentiation in Arabidopsis root meristems.\u00a0Development<\/em><\/span><\/strong>,<\/span> Vol. 144: pp. 272-280.<\/a><\/p>\n

Yeon Hee Kang\u00a0and Christian S. Hardtke (2016):\u00a0Arabidopsis MAKR5 is a positive effector of BAM3-dependent CLE45 signaling.\u00a0EMBO Reports<\/em><\/span><\/strong>,<\/span> Vol. 17: pp. 1145-1154.<\/a><\/p>\n

David Pacheco-Villalobos, Sara M. D\u00edaz-Moreno, Alja van der Schuren, Takayuki Tamaki, Yeon Hee Kang, Bojan Gujas, Ondrej Novak, Nina Jaspert, Zhenni Li, Sebastian Wolf, Claudia Oecking, Karin Ljung, Vincent Bulone, and Christian S. Hardtke (2016):\u00a0The Effects of High Steady State Auxin Levels on Root Cell Elongation in Brachypodium.\u00a0The Plant Cell<\/em><\/span><\/strong>,<\/span> Vo. 28: pp. 1009-1024.<\/a><\/p>\n

Antia Rodriguez-Villalon, Bojan Gujas,\u00a0Ringo van Wijk, Teun Munnik and\u00a0Christian S. Hardtke (2015):\u00a0Primary root protophloem differentiation requires balanced phosphatidylinositol-4,5-biphosphate levels and systemically affects root branching.<\/strong> Development<\/em><\/strong><\/strong>,<\/span> doi: 10.1242\/dev.118364.<\/a><\/p>\n

Antia Rodriguez-Villalon, Bojan Gujas, Yeon Hee Kang, Alice Breda, Pietro Cattaneo, Stephen Depuydt and Christian S. Hardtke\u00a0(2014):\u00a0A Molecular Genetic Framework For Protophloem Formation.\u00a0Proceedings of the National Academy of Sciences U.S.A.<\/em><\/strong><\/span><\/strong>,<\/span>\u00a0Vol. 111: pp. 11551-11556.<\/a><\/p>\n

Antia Rodriguez-Villalon\u00a0and Christian S. Hardtke (2014):\u00a0Auxin and its henchmen: hormonal crosstalk in root growth and development. <\/strong>in Auxin and its Role in Plant Development<\/em><\/strong>,<\/span> Springer, 2014.<\/a><\/p>\n

Stephen Depuydt, Antia Rodriguez-Villalon, Luca Santuari, C\u00e9line Wyser-Rmili, Laura Ragni and Christian S. Hardtke (2013):\u00a0Suppression of protophloem differentiation and root meristem growth in Arabidopsis by CLE45 requires the receptor-like kinase BAM3.<\/strong>\u00a0Proceedings of the National Academy of Sciences U.S.A.<\/strong><\/em>,<\/span> Vol. 110: pp. 7074-7079.<\/a><\/p>\n

David Pacheco-Villalobos,\u00a0\u00a0Martial Sankar, Karin Ljung\u00a0and Christian S. Hardtke (2013):\u00a0Disturbed local auxin homeostasis enhances cellular anisotropy and reveals alternative wiring of auxin-ethylene crosstalk in\u00a0Brachypodium distachyon<\/em>\u00a0seminal roots<\/strong>.<\/strong>\u00a0PLoS Genetics<\/strong><\/em>,<\/span> doi:10.1371\/journal.pgen.1003564.<\/a><\/p>\n

Luca Santuari, Emanuele Scacchi, Antia Rodriguez-Villalon, Paula Salinas, Esther M.N. Dohmann, Geraldine Brunoud, Teva Vernoux, Richard S. Smith and Christian S. Hardtke (2011):\u00a0Positional information by differential endocytosis splits auxin response to drive Arabidopsis root meristem growth.<\/strong>\u00a0Current Biology<\/strong><\/em>,<\/span> Vol. 21: pp. 1918-1923.<\/a><\/p>\n

Stephen Depuydt and\u00a0Christian S. Hardtke (2011):\u00a0Hormone Signalling Crosstalk in Plant Growth Regulation.<\/strong>\u00a0Current Biology<\/span><\/strong><\/em>,<\/span> Vol. 21: pp. R365-R373.<\/a><\/p>\n

Emanuele Scacchi, Paula Salinas, Bojan Gujas, Luca Santuari, Naden Krogan, Laura Ragni, Thomas Berleth and Christian S. Hardtke (2010):\u00a0Spatio-temporal sequence of cross-regulatory events in root meristem growth.<\/strong>\u00a0Proceedings of the National Academy of Sciences U.S.A.<\/strong><\/em>,<\/span> Vol. 107: pp. 22734-22739.<\/a><\/p>\n

Emanuele Scacchi, Karen S. Osmont, Julien Beuchat, Paula Salinas, Marisa Navarrete-G\u00f3mez, Marina Trigueros, Cristina Ferr\u00e1ndiz and Christian S. Hardtke (2009):\u00a0Dynamic, auxin-responsive plasma membrane to nucleus movement of Arabidopsis BRX.\u00a0<\/strong>Development<\/em><\/strong>,<\/span>\u00a0Vol. 136: pp. 2059-2067.<\/a><\/p>\n

Richard Sibout, Poornima Sukuma, Chamari Hettiarachchi, Magnus Holm, Gloria K. Muday and Christian S. Hardtke (2006):\u00a0Opposite root growth phenotypes of hy5<\/em> vs. hy5 hyh<\/em> mutants correlate with increased constitutive auxin signaling.\u00a0PLoS Genetics<\/span><\/em><\/strong>,<\/span> Vol. 2: pp. 1898-1911, e202.<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

Vascular differentiation and its relation to plant hormone pathways has become a major focus of our lab over the years. The importance of the vascular system for plant development cannot … <\/p>\n","protected":false},"author":1001,"featured_media":0,"parent":7,"menu_order":0,"comment_status":"open","ping_status":"closed","template":"","meta":{"_seopress_robots_primary_cat":"","_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","footnotes":""},"class_list":["post-1133","page","type-page","status-publish"],"_links":{"self":[{"href":"https:\/\/wp.unil.ch\/hardtkelab\/wp-json\/wp\/v2\/pages\/1133","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wp.unil.ch\/hardtkelab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wp.unil.ch\/hardtkelab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wp.unil.ch\/hardtkelab\/wp-json\/wp\/v2\/users\/1001"}],"replies":[{"embeddable":true,"href":"https:\/\/wp.unil.ch\/hardtkelab\/wp-json\/wp\/v2\/comments?post=1133"}],"version-history":[{"count":0,"href":"https:\/\/wp.unil.ch\/hardtkelab\/wp-json\/wp\/v2\/pages\/1133\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/wp.unil.ch\/hardtkelab\/wp-json\/wp\/v2\/pages\/7"}],"wp:attachment":[{"href":"https:\/\/wp.unil.ch\/hardtkelab\/wp-json\/wp\/v2\/media?parent=1133"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}