{"id":690,"date":"2021-04-08T15:09:34","date_gmt":"2021-04-08T13:09:34","guid":{"rendered":"https:\/\/wp.unil.ch\/gtf\/?page_id=690"},"modified":"2026-04-02T14:08:58","modified_gmt":"2026-04-02T12:08:58","slug":"utilities","status":"publish","type":"page","link":"https:\/\/wp.unil.ch\/gtf\/utilities\/","title":{"rendered":"Utilities"},"content":{"rendered":"\n
Fragment Analyzer Guidelines<\/strong><\/summary>\n

– Fill out the sample submission form (LINK to excel file<\/a>)<\/p>\n\n\n\n

– Save sample submission form with file name YYYYMMDD_PIName_UserName<\/p>\n\n\n\n

– Put samples in a plastic bag with your name, PI, Institute, sample type (RNA, gDNA, NGS library, amplicon\u2026), date, High or Standard Sensitivity Assay<\/p>\n\n\n\n

– RNA : Please use 0.2 ml 8-strip tubes, number tubes, send email with sample submission form to gtf-fa@unil.ch<\/a>. Drop bag in -80, red box<\/p>\n\n\n\n

– DNA (NGS libraries, gDNA, fragmented gDNA, amplicons): Please use 0.2 ml 8-strip tubes, number tubes, send email with sample submission form to gtf-fa@unil.ch<\/a>.<\/p>\n\n\n\n

– Give bag to someone from GTF (or drop the bag in our -80 outside the Facility if the lab is closed). If you want both Frag. Analyzer + Qubit we need a minimum of 5 \u00b5l.<\/p>\n\n\n\n

– If the concentration of your sample is between the Standard and High Sensitivity ranges, please dilute it for High Sensitivity Assay!<\/p>\n\n\n\n

– For \u2265 36 samples you can ask for bulk price (standard price -25%). In this case prepare the samples in a 96 well PCR plate, filled by lanes (A1-A12, B1-B12 etc) with exactly 2 \u00b5l volume<\/p>\n\n\n\n

<\/div>\n\n\n\n
RNA<\/strong> Aliquot of 3\u00b5l to 4\u00b5l<\/td>High Sensitivity<\/strong><\/td>Standard Sensitivity<\/strong><\/td><\/tr>
Concentration<\/td>1ng – 5ng\/\u00b5l<\/td>30 \u2013 500ng\/\u00b5l<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
DNA<\/strong> Aliquot of 3\u00b5l to 4\u00b5l<\/td>High Sensitivity<\/strong><\/td>Standard Sensitivity<\/strong><\/td><\/tr>
NGS lib., fragmented DNA<\/td>0.1 \u2013 5ng\/\u00b5l<\/td>20 \u2013 100ng\/\u00b5l<\/td><\/tr>
Amplicons (1 \u2013 6000bp)
Single peak or few peaks<\/td>		0.1 \u2013 2ng\/\u00b5l<\/td>5 \u2013 20ng\/\u00b5l<\/td><\/tr>
gDNA (1 \u2013 50000bp)<\/td>0.5 \u2013 2ng\/\u00b5l<\/td>NA<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
\"\"<\/figure>\n\n\n\n

<\/p>\n<\/details>\n\n\n\n

Dropbox GTF<\/strong><\/summary>\n

The dropbox is designed to facilitate the transfer of your samples to the GTF laboratory in Dorigny<\/p>\n\n\n\n

Please note that it does not replace the standard submission procedures<\/strong>
For Fragment Analyzer submissions, refer to the dedicated tutorial above
For library preparation and sequencing, projects must first be discussed with the GTF staff and registered in our LIMS<\/a><\/p>\n\n\n\n

Please fill the file: GTF-Dropbox.xls<\/a><\/p>\n\n\n\n

Send it by email to dropbox-gtf@unil.ch<\/a><\/p>\n\n\n\n

We recover samples on Tuesdays <\/strong>and Thursdays <\/strong>early morning
Please send the email before 16H<\/strong> the day before to ensure we know your samples are waiting<\/p>\n\n\n\n

Agora Dropbox<\/summary>\n

Rue du Bugnon 25A,1005 Lausanne – Second floor<\/p>\n\n\n\n