{"id":381,"date":"2020-03-10T15:55:18","date_gmt":"2020-03-10T14:55:18","guid":{"rendered":"http:\/\/wp.unil.ch\/gtf\/?page_id=381"},"modified":"2026-06-29T12:51:39","modified_gmt":"2026-06-29T10:51:39","slug":"single-cell-experiments-10x","status":"publish","type":"page","link":"https:\/\/wp.unil.ch\/gtf\/single-cell-experiments-10x\/","title":{"rendered":"Single Cell"},"content":{"rendered":"\n<h1 class=\"wp-block-heading\"><strong>Our Service Workflows<\/strong><\/h1>\n\n\n\n<p class=\"wp-block-paragraph\">Our Facility offers a broad range of single-cell applications, providing expert knowledge, accessibility, and personalized support.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">We continuously update our methods and instruments in collaboration with our users, ensuring that we stay in tune with the evolving needs of the research community.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">We support end-to-end :<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>10x Genomics Chromium System (droplet-based) for fresh and fixed cells assays<\/li>\n\n\n\n<li>Parse Bioscience Evercode (plate-based)<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\">We can also support Waters Bioscience BD Rhapsody assays (please contact us for more information).<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Other user-made library types can be sequenced at the Facility.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Users are responsible for the preparation of their single cell suspensions and ensuring that sample quality meets the required standards. We can provide you with recommendations.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Our Facility takes care of your cell suspensions provided at one of our 3 locations (please inquire).<\/p>\n\n\n\n<figure class=\"wp-block-table aligncenter\"><table class=\"has-fixed-layout\"><tbody><tr><td><strong>Genopode building<\/strong> <br>Dorigny lab 1016<br>in GTF Facility<\/td><td><strong>Agora building<\/strong> <br>CHUV lab 227 <br>in FCF Facility<\/td><td><strong>Biopole SE-C building<\/strong> <br>Epalinges lab 136 <br>in FCF Facility<\/td><\/tr><tr><td><img loading=\"lazy\" decoding=\"async\" width=\"592\" height=\"717\" class=\"wp-image-2200\" style=\"width: 150px\" src=\"https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/chromium.png\" alt=\"chromium@GTF\" srcset=\"https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/chromium.png 592w, https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/chromium-248x300.png 248w\" sizes=\"auto, (max-width: 592px) 100vw, 592px\" \/><\/td><td><img loading=\"lazy\" decoding=\"async\" width=\"730\" height=\"807\" class=\"wp-image-2205\" style=\"width: 150px\" src=\"https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/room.png\" alt=\"chromium@CHUV\" srcset=\"https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/room.png 730w, https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/room-271x300.png 271w\" sizes=\"auto, (max-width: 730px) 100vw, 730px\" \/><\/td><td><img loading=\"lazy\" decoding=\"async\" width=\"492\" height=\"656\" class=\"wp-image-2198\" style=\"width: 150px\" src=\"https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/bench2.png\" alt=\"Chromium@Epalinges\" srcset=\"https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/bench2.png 492w, https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/bench2-225x300.png 225w\" sizes=\"auto, (max-width: 492px) 100vw, 492px\" \/><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<h1 class=\"wp-block-heading\"><strong>Schedule An Experiment<\/strong><\/h1>\n\n\n\n<p class=\"wp-block-paragraph\">Samples should be processed as quickly as possible after the cell sorting. Accordingly, experiments must be carefully scheduled with our staff.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Please&nbsp;<a href=\"mailto:contactGTF@unil.ch\">contactGTF@unil.ch<\/a> &nbsp;as early as possible when designing your project and provide us with as much information about your project by filling the following form :<\/p>\n\n\n\n<div class=\"wp-block-file\"><a id=\"wp-block-file--media-66c8a4e1-5b24-4e53-b95c-5c248143e178\" href=\"https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/gtf_singlecell_form_v02.docx\">GTF_SingleCell_Form_v02<\/a><a href=\"https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/gtf_singlecell_form_v02.docx\" class=\"wp-block-file__button wp-element-button\" download aria-describedby=\"wp-block-file--media-66c8a4e1-5b24-4e53-b95c-5c248143e178\">Download<\/a><\/div>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<h1 class=\"wp-block-heading\"><strong>Sample Preparation<\/strong><\/h1>\n\n\n\n<h3 class=\"wp-block-heading\">Cells or nuclei preparation is user\u2019s responsibility!<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">The cells\/nuclei suspension quality is crucial for a successful single cell project. Cells\/Nuclei must be perfectly dissociated, their density accurately quantified, and viability must be above 70% (for transcriptomics applications). Nuclei preparation is even more challenging since viability check does not apply .<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Any manipulation (e.g. concentration by centrifugation) or waiting time can impact cells\/nuclei quantity and quality. In particular, cell count and viability output metrics from cell sorters cannot be taken for granted. It is more than highly recommended that a final quality control step is performed on the&nbsp;very final material&nbsp;you provide us. You may want take advantage of our cell counter <a href=\"https:\/\/logosbio.com\/luna-fx7\/\" target=\"_blank\" rel=\"noreferrer noopener\">Luna-FX7<\/a>&nbsp;which relies on AO\/PI staining to accurately distinguish cells from debris.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Failure to provide good quality starting material will inevitably result in suboptimal, sometimes useless, results. Data quality can only be assessed after sequencing, meaning that expensive single cell and sequencing reagents are engaged, whatever the outcome is.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>In any case, we strongly recommend that pilot experiments are performed in order to validate the cell preparation procedure.<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Popular links for sample preparation&nbsp;:<\/h3>\n\n\n\n<h5 class=\"wp-block-heading\"><span style=\"text-decoration: underline\">10X Genomics<\/span><\/h5>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.10xgenomics.com\/support\"><strong>Generic Tech support<\/strong><\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/assets.10xgenomics.com\/m\/7122b03c55bc48ca\/original\/CG00053_Handbook_CellPreparation_SingleCellProtocols.pdf\"><strong>Cell Preparation for single cell protocols<\/strong><\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/cdn.10xgenomics.com\/image\/upload\/v1660831100\/support-documents\/CG000093_Demonstrated_Protocol_DeadCellRemoval_RevC.pdf\"><strong>Removal of dead cells<\/strong><\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/cdn.10xgenomics.com\/image\/upload\/v1775599228\/user-guides-pdfs\/CG000505_Chromium_Nuclei_Isolation_Kit_UG_RevB.pdf\"><strong>Nuclei preparation for single cell protocols<\/strong><\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/cdn.10xgenomics.com\/image\/upload\/v1761673488\/support-documents\/CG000783_DemonstratedProtocol_TissueFixationDissociation_GEM-X_Flex_GeneExpression_RevC.pdf\"><strong>Tissu Fixation and dissociation protocol<\/strong><\/a><\/li>\n<\/ul>\n\n\n\n<h5 class=\"wp-block-heading\"><span style=\"text-decoration: underline\">Parse<\/span><\/h5>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/support.parsebiosciences.com\/hc\/en-us\/sections\/39206906338964-Sample-Preparation-Protocols\"><strong>Sample preparation for single cell protocols<\/strong><\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.parsebiosciences.com\/products\/evercode-fixation\/\"><strong>Fixation protocols<\/strong><\/a><\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\"> <\/p>\n\n\n\n<p class=\"wp-block-paragraph\"> <\/p>\n\n\n\n<h1 class=\"wp-block-heading\"><strong>Technology description<\/strong><\/h1>\n\n\n\n<h3 class=\"wp-block-heading\">10x Genomics Chromium System (droplet-based)<\/h3>\n\n\n\n<h5 class=\"wp-block-heading\"><span style=\"text-decoration: underline\">For Fresh cells:<\/span><\/h5>\n\n\n\n<p class=\"wp-block-paragraph\">In addition to fresh material, cryopreserved cells are a suitable input if they retain high viability at thawing.&nbsp;<\/p>\n\n\n\n<h6 class=\"wp-block-heading\">3\u2019 or 5\u2019 GEM-X with multiomic capabilities&nbsp;:<\/h6>\n\n\n\n<ul class=\"wp-block-list\">\n<li>3\u2019 gene expression with cell surface protein<\/li>\n\n\n\n<li>5\u2019 gene expression with BCR\/TCR, cell surface protein, antigen specificity and CRISPR screening<\/li>\n<\/ul>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"613\" height=\"230\" src=\"https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/10x.png\" alt=\"10x\" class=\"wp-image-2195\" srcset=\"https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/10x.png 613w, https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/10x-300x113.png 300w\" sizes=\"auto, (max-width: 613px) 100vw, 613px\" \/><\/figure>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Cells\/nuclei, fresh, frozen tissues or organoids<\/li>\n\n\n\n<li>Recovery of up to 20\u2019000 cells\/sample (for 30\u2019000 cells input)<\/li>\n\n\n\n<li>Possibility for multiplexing with BioLegend hashtags (see TotalSeq product section on BioLegend\u2019s website)<\/li>\n\n\n\n<li>Built-in multiplexing capabilities for fresh cells<\/li>\n<\/ul>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"600\" height=\"199\" src=\"https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/10x_plate.png\" alt=\"10x plate\" class=\"wp-image-2194\" srcset=\"https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/10x_plate.png 600w, https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/10x_plate-300x100.png 300w\" sizes=\"auto, (max-width: 600px) 100vw, 600px\" \/><\/figure>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Recovery up to 20\u2019000 cells\/ 4 samples (5\u2019000 recovered cells per sample for 8\u2019000 cells per sample input)<\/li>\n<\/ul>\n\n\n\n<h6 class=\"wp-block-heading\">GEM-X Epi Multiome&nbsp;:<\/h6>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Some cells states are nearly identical at the transcript level but differ significantly in their epigenitic memory or regulatory landscape<\/li>\n\n\n\n<li>3\u2019 gene expression and chromatin accessibility in the same cell<\/li>\n<\/ul>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"433\" src=\"https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/10xb-1024x433.png\" alt=\"10xb\" class=\"wp-image-2196\" srcset=\"https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/10xb-1024x433.png 1024w, https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/10xb-300x127.png 300w, https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/10xb-768x325.png 768w, https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/10xb.png 1147w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"wp-element-caption\"><em>Image adapted from 10x Genomics<\/em><\/figcaption><\/figure>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/cdn.10xgenomics.com\/image\/upload\/v1776987935\/support-documents\/CG000365_DemonstratedProtocol_NucleiIsolation_ATAC_GEX_Sequencing-Rev_F.pdf\">New Nuclei isolation kit with RNAase inhibitor v2<\/a><\/li>\n\n\n\n<li>Recovery\u00a0: maximum 20\u2019000 nuclei\/sample<\/li>\n<\/ul>\n\n\n\n<h5 class=\"wp-block-heading\"><span style=\"text-decoration: underline\">For Fixed cell : Flex v2 Apex<\/span><\/h5>\n\n\n\n<p class=\"wp-block-paragraph\">Enables to preserve the sample biology through fixation (PFA) at sample collection.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">A probe-based solution targeting 18\u2019000 transcripts (human and mouse) for the whole transcriptome gene expression (other custom probes available).<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"1920\" height=\"707\" src=\"https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/flex-fam_howflex_workflow_1200x474.png\" alt=\"flex fam howflex workflow 1200x474\" class=\"wp-image-2244\" srcset=\"https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/flex-fam_howflex_workflow_1200x474.png 1920w, https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/flex-fam_howflex_workflow_1200x474-300x110.png 300w, https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/flex-fam_howflex_workflow_1200x474-1024x377.png 1024w, https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/flex-fam_howflex_workflow_1200x474-768x283.png 768w, https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/flex-fam_howflex_workflow_1200x474-1536x566.png 1536w\" sizes=\"auto, (max-width: 1920px) 100vw, 1920px\" \/><figcaption class=\"wp-element-caption\"><em>10X Genomics &#8211; flex fam howflex workflow <\/em><\/figcaption><\/figure>\n\n\n\n<ul class=\"wp-block-list\">\n<li>In-line multiplexing for 1 up to 384 samples per emulsion for ultimate flexibility<\/li>\n\n\n\n<li>Cells\/nuclei, fresh, frozen and FFPE tissues, organoids or fixed whole blood<\/li>\n\n\n\n<li>Multiomics capabilities : cell surface protein, intracellular protein, CRISPR screening<\/li>\n\n\n\n<li>Input as low as 100\u2019000 cells\/sample (less is possible, please inquire)<\/li>\n\n\n\n<li>Excluded genes :\n<ul class=\"wp-block-list\">\n<li>TCR &amp; IG variable\/joining regions<\/li>\n\n\n\n<li>Ribosomal &amp; mitochondrial ribosomal proteins<\/li>\n\n\n\n<li>Read-through genes<\/li>\n\n\n\n<li>KIR &amp; HLA genes (high allelic diversity)<\/li>\n\n\n\n<li>Non-coding RNA<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Parse Bioscience Evercode (plate-based)<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">Enables to preserve the sample biology through mild fixation at sample collection.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Whole transcriptome gene expression (oligoT + random primers) makes it suitable for any species.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Split-pool combinatorial barcoding (1 to 384 samples).<\/p>\n\n\n\n<figure class=\"wp-block-image size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"393\" src=\"https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/parse-1-1024x393.png\" alt=\"parse\" class=\"wp-image-2247\" style=\"width:834px;height:auto\" srcset=\"https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/parse-1-1024x393.png 1024w, https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/parse-1-300x115.png 300w, https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/parse-1-768x295.png 768w, https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/parse-1-1536x590.png 1536w, https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/parse-1.png 1784w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"wp-element-caption\"><em>Parse Biosciences 2024<\/em><\/figcaption><\/figure>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Mild fixation for 100\u2019000-300\u2019000 cells\/sample and even less with low input fixation kit<\/li>\n\n\n\n<li>Fresh cells\/nuclei, FFPE tissues, fresh-frozen and fixed-frozen samples<\/li>\n\n\n\n<li>Multiomics capabilities: TCR, BCR, CRISPR screening<\/li>\n\n\n\n<li>Different sizes of kit :\n<ul class=\"wp-block-list\">\n<li>Evercode WT \u2013 1-48 samples &#8211; recovery 100K cells total<\/li>\n\n\n\n<li>Evercode Mega \u2013 1-384 samples &#8211; recovery 1M cells total<\/li>\n\n\n\n<li>Evercode Penta \u2013 1-384 samples &#8211; recovery 5M cells total<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<h3 class=\"wp-block-heading\">WATERS Biosciences (Former BD) (microwells-based)<\/h3>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"304\" src=\"https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/waters1-1024x304.png\" alt=\"waters1\" class=\"wp-image-2207\" srcset=\"https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/waters1-1024x304.png 1024w, https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/waters1-300x89.png 300w, https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/waters1-768x228.png 768w, https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/waters1.png 1205w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"wp-element-caption\"><br><em>Image adapted from Waters Biosciences<\/em><\/figcaption><\/figure>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Whole Transcriptome<\/li>\n\n\n\n<li>Multiomics capabilities : TCR, BCR, CITE-Seq, Protein, ATACseq, Ag specificity<\/li>\n\n\n\n<li>This technology is not directly available at the GTF but inquire<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Spatial Transcriptomics<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">GTF teams up with another facility to propose spatial transcriptomics and proteomics analysis (please inquire).<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<h1 class=\"wp-block-heading\"><strong>Sequencing<\/strong><\/h1>\n\n\n\n<p class=\"wp-block-paragraph\">We perform 10X library sequencing on our Element Biosciences or Illumina sequencer, depending on the output required.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Depth can vary a lot depending on your need :<\/p>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td><strong>Assay<\/strong><\/td><td><strong>Minimum reads per cell recommended<\/strong><\/td><\/tr><tr><td>10x 3\u2019 or 5\u2019 GEX<\/td><td>20\u2019000<\/td><\/tr><tr><td>10x cell surface protein<\/td><td>5\u2019000<\/td><\/tr><tr><td>10x BCR\/TCR<\/td><td>5\u2019000<\/td><\/tr><tr><td>10x CRISPR<\/td><td>5\u2019000<\/td><\/tr><tr><td>10x ATACseq<\/td><td>25\u2019000<\/td><\/tr><tr><td>10x FLEX V2<\/td><td>10\u2019000<\/td><\/tr><tr><td>PARSE V4<\/td><td>10\u2019000<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\">A good practice is to request a single sequencing lane (600M) to start with. Further sequencing can then be requested after quality control for validating cell number, evaluating transcription saturation, and inspecting the presence of some genes of interest.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Pricing for sequencing is variable and is determined by multiple factors including :<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Technology you choose<\/li>\n\n\n\n<li>Number of samples<\/li>\n\n\n\n<li>Number of cells you aims to target per sample<\/li>\n\n\n\n<li>Read depth per cell<\/li>\n\n\n\n<li>Optional extensions<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<h1 class=\"wp-block-heading\"><strong>Data Analysis<\/strong><\/h1>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Primary analysis<\/strong> with dedicated pipelines is included with our single cell service.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">It may also be requested for <strong>user made libraries<\/strong> sequenced at GTF. Please contact us concerning pricing.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Note that analysis with <strong>custom genome<\/strong> reference (presence of a transgene, like GFP) or non-model organisms is also possible. Please contact us for further information and associated fees.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">10x Genomics &#8211; Cell Ranger analysis pipeline<\/h3>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"812\" height=\"372\" src=\"https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/cellranger.png\" alt=\"cellranger\" class=\"wp-image-2199\" srcset=\"https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/cellranger.png 812w, https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/cellranger-300x137.png 300w, https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/cellranger-768x352.png 768w\" sizes=\"auto, (max-width: 812px) 100vw, 812px\" \/><figcaption class=\"wp-element-caption\"><br><em>Example Cellranger pipeline output &#8211; Image adapted from 10x Genomics<\/em><\/figcaption><\/figure>\n\n\n\n<h3 class=\"wp-block-heading\">Parse Biosciences \u2013 Parse analysis pipeline<\/h3>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"898\" height=\"582\" src=\"https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/from-parse-bioscience.png\" alt=\"from parse bioscience\" class=\"wp-image-2257\" srcset=\"https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/from-parse-bioscience.png 898w, https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/from-parse-bioscience-300x194.png 300w, https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/from-parse-bioscience-768x498.png 768w\" sizes=\"auto, (max-width: 898px) 100vw, 898px\" \/><figcaption class=\"wp-element-caption\"><em>From Parse Biosciences<\/em><\/figcaption><\/figure>\n\n\n\n<h3 class=\"wp-block-heading\">BD Rhapsody\u2122 Sequence Analysis Pipeline\u00a0<\/h3>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"1040\" height=\"390\" src=\"https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/bd-rhapsody.png\" alt=\"bd rhapsody\" class=\"wp-image-2256\" srcset=\"https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/bd-rhapsody.png 1040w, https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/bd-rhapsody-300x113.png 300w, https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/bd-rhapsody-1024x384.png 1024w, https:\/\/wp.unil.ch\/gtf\/files\/2026\/06\/bd-rhapsody-768x288.png 768w\" sizes=\"auto, (max-width: 1040px) 100vw, 1040px\" \/><figcaption class=\"wp-element-caption\"><em>BD Rhapsody<\/em><\/figcaption><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Our Service Workflows Our Facility offers a broad range of single-cell applications, providing expert knowledge, accessibility, and personalized support. 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