{"id":293,"date":"2013-06-26T16:40:07","date_gmt":"2013-06-26T14:40:07","guid":{"rendered":"http:\/\/wp.unil.ch\/geldnerlab\/?page_id=293"},"modified":"2020-03-24T15:56:21","modified_gmt":"2020-03-24T14:56:21","slug":"protocols","status":"publish","type":"page","link":"https:\/\/wp.unil.ch\/geldnerlab\/resources-and-protocols\/protocols\/","title":{"rendered":"Protocols"},"content":{"rendered":"<p><img alt=\"\" loading=\"lazy\" decoding=\"async\" class=\"wp-image-924 aligncenter\" src=\"https:\/\/wp.unil.ch\/geldnerlab\/files\/2018\/07\/Casparian-strip-300x228.png\" alt=\"\" width=\"166\" height=\"126\" srcset=\"https:\/\/wp.unil.ch\/geldnerlab\/files\/2018\/07\/Casparian-strip-300x228.png 300w, https:\/\/wp.unil.ch\/geldnerlab\/files\/2018\/07\/Casparian-strip-768x583.png 768w, https:\/\/wp.unil.ch\/geldnerlab\/files\/2018\/07\/Casparian-strip-1024x778.png 1024w, https:\/\/wp.unil.ch\/geldnerlab\/files\/2018\/07\/Casparian-strip.png 1327w\" sizes=\"auto, (max-width: 166px) 100vw, 166px\" \/><\/p>\n<p style=\"text-align: center\"><a href=\"https:\/\/wp.unil.ch\/geldnerlab\/files\/2018\/07\/ClearSee-protocol-for-staining-cell-walls.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">ClearSee-adapted protocol for cell wall staining<\/a><\/p>\n<p>&nbsp;<\/p>\n<p style=\"text-align: center\"><a href=\"https:\/\/wp.unil.ch\/geldnerlab\/files\/2013\/06\/Propidium-Iodide2.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-297 aligncenter\" src=\"https:\/\/wp.unil.ch\/geldnerlab\/files\/2013\/06\/Captura-de-pantalla-2013-06-26-a-las-16.35.27.png\" alt=\"Captura de pantalla 2013-06-26 a las 16.35.27\" width=\"104\" height=\"140\" \/><\/a><a href=\"https:\/\/wp.unil.ch\/geldnerlab\/files\/2013\/06\/Propidium-Iodide1.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Propidium Iodide<\/a><\/p>\n<p>&nbsp;<\/p>\n<p style=\"text-align: center\"><a href=\"https:\/\/wp.unil.ch\/geldnerlab\/files\/2013\/07\/Autofluorescence-Clearing.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-298 aligncenter\" src=\"https:\/\/wp.unil.ch\/geldnerlab\/files\/2013\/06\/Captura-de-pantalla-2013-06-26-a-las-16.35.37.png\" alt=\"Captura de pantalla 2013-06-26 a las 16.35.37\" width=\"103\" height=\"128\" \/><\/a><a href=\"https:\/\/wp.unil.ch\/geldnerlab\/files\/2013\/07\/Autofluorescence-Clearing.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Lignin visualisation using AutoFluorescence (Clearing)<\/a><\/p>\n<p>&nbsp;<\/p>\n<p style=\"text-align: center\"><a href=\"https:\/\/wp.unil.ch\/geldnerlab\/files\/2013\/07\/Fluorol-Yellow-staining.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-299 aligncenter\" src=\"https:\/\/wp.unil.ch\/geldnerlab\/files\/2013\/06\/Captura-de-pantalla-2013-06-26-a-las-16.35.47.png\" alt=\"Captura de pantalla 2013-06-26 a las 16.35.47\" width=\"99\" height=\"131\" \/><\/a><a href=\"https:\/\/wp.unil.ch\/geldnerlab\/files\/2013\/07\/Fluorol-Yellow-staining.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Fluorol Yellow Staining for Suberin (lactic acid-based)<\/a><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p style=\"text-align: center\"><img alt=\"\" loading=\"lazy\" decoding=\"async\" class=\"wp-image-1070 aligncenter\" src=\"https:\/\/wp.unil.ch\/geldnerlab\/files\/2020\/02\/FYCW-152x300.png\" alt=\"\" width=\"98\" height=\"193\" srcset=\"https:\/\/wp.unil.ch\/geldnerlab\/files\/2020\/02\/FYCW-152x300.png 152w, https:\/\/wp.unil.ch\/geldnerlab\/files\/2020\/02\/FYCW.png 520w\" sizes=\"auto, (max-width: 98px) 100vw, 98px\" \/>\u00a0<span><br \/>\n<\/span><a href=\"https:\/\/wp.unil.ch\/geldnerlab\/files\/2020\/03\/Methanol.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Methanol based combined Florol Yellow and Calcofluor white staining to visualise Arabidopsis root suberin and cell walls<\/a><\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"font-size: x-small\">Images Copyright:<br \/>\n(PI; AF; FYS) were taken from Proc Natl Acad Sci U S A. 2012 Jun 19; 109(25): 10101-6.<br \/>\n(CP) Robetas Ursache<\/span><\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>ClearSee-adapted protocol for cell wall staining &nbsp; Propidium Iodide &nbsp; Lignin visualisation using AutoFluorescence (Clearing) &nbsp; Fluorol Yellow Staining for Suberin (lactic acid-based) &nbsp; &nbsp; \u00a0 Methanol based combined Florol &hellip; <\/p>\n","protected":false},"author":108,"featured_media":0,"parent":249,"menu_order":2,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_robots_primary_cat":"","_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","footnotes":""},"class_list":["post-293","page","type-page","status-publish"],"_links":{"self":[{"href":"https:\/\/wp.unil.ch\/geldnerlab\/wp-json\/wp\/v2\/pages\/293","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wp.unil.ch\/geldnerlab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wp.unil.ch\/geldnerlab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wp.unil.ch\/geldnerlab\/wp-json\/wp\/v2\/users\/108"}],"replies":[{"embeddable":true,"href":"https:\/\/wp.unil.ch\/geldnerlab\/wp-json\/wp\/v2\/comments?post=293"}],"version-history":[{"count":0,"href":"https:\/\/wp.unil.ch\/geldnerlab\/wp-json\/wp\/v2\/pages\/293\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/wp.unil.ch\/geldnerlab\/wp-json\/wp\/v2\/pages\/249"}],"wp:attachment":[{"href":"https:\/\/wp.unil.ch\/geldnerlab\/wp-json\/wp\/v2\/media?parent=293"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}