{"id":1793,"date":"2025-11-10T11:49:30","date_gmt":"2025-11-10T10:49:30","guid":{"rendered":"https:\/\/wp.unil.ch\/fcf\/?page_id=1793"},"modified":"2025-12-12T11:27:28","modified_gmt":"2025-12-12T10:27:28","slug":"cytek-aurora-3-agora","status":"publish","type":"page","link":"https:\/\/wp.unil.ch\/fcf\/cytek-aurora-3-agora\/","title":{"rendered":"Cytek Aurora 3 (Agora Cancer Center)"},"content":{"rendered":"\n<p>The Cytek Aurora 3 is a 5 lasers spectral cytometers.<\/p>\n\n\n\n<figure class=\"wp-block-image\"><img alt=\"\" loading=\"lazy\" decoding=\"async\" width=\"326\" height=\"154\" src=\"https:\/\/wp.unil.ch\/fcf\/files\/2020\/04\/download.jpeg\" alt=\"\" class=\"wp-image-809\" srcset=\"https:\/\/wp.unil.ch\/fcf\/files\/2020\/04\/download.jpeg 326w, https:\/\/wp.unil.ch\/fcf\/files\/2020\/04\/download-300x142.jpeg 300w\" sizes=\"auto, (max-width: 326px) 100vw, 326px\" \/><\/figure>\n\n\n\n<p><\/p>\n\n\n\n<p>Spectral Flow Cytometry provides an alternative way to measure fluorescence emission. Contrary to conventional flow cytometry which measures a limited bandwidth of the emission spectrum of each fluorochrome, spectral flow cytometry measures the entire spectral signature emitted from each of the 5 lasers. This enables a much more in depth mapping of each fluorochrome\/fluorescent protein emission and allows simultaneous detection and discrimination of fluorochromes that were not possible before.<\/p>\n\n\n\n<p>The Aurora has 64 detectors which provides a signal per fluorochrome similar to the example below:<\/p>\n\n\n\n<figure class=\"wp-block-image\"><img decoding=\"async\" src=\"https:\/\/wp.unil.ch\/fcf\/files\/2020\/04\/signature.png\" alt=\"\" \/><\/figure>\n\n\n\n<p><\/p>\n\n\n\n<p>As another example, PE will give this signal :<\/p>\n\n\n\n<figure class=\"wp-block-image\"><img alt=\"\" loading=\"lazy\" decoding=\"async\" width=\"1091\" height=\"371\" src=\"https:\/\/wp.unil.ch\/fcf\/files\/2020\/09\/PEspectral.png\" alt=\"\" class=\"wp-image-879\" srcset=\"https:\/\/wp.unil.ch\/fcf\/files\/2020\/09\/PEspectral.png 1091w, https:\/\/wp.unil.ch\/fcf\/files\/2020\/09\/PEspectral-300x102.png 300w, https:\/\/wp.unil.ch\/fcf\/files\/2020\/09\/PEspectral-1024x348.png 1024w, https:\/\/wp.unil.ch\/fcf\/files\/2020\/09\/PEspectral-768x261.png 768w\" sizes=\"auto, (max-width: 1091px) 100vw, 1091px\" \/><\/figure>\n\n\n\n<p><\/p>\n\n\n\n<p>There is two kind of signal or &#8220;<strong>signature<\/strong>&#8221; :<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Unique Signatures :<\/strong> The fluorochrome can be easily discriminated among others. Each unique signature can be read simultaneously on the machine. FITC and GFP, APC and Alexa Fluor 647 have unique signatures.<\/li>\n\n\n\n<li><strong>Similar Signatures :<\/strong> These fluorochromes have signatures indistinguishable from one another. PE Texas Red and PE-CF594 have similar signatures. In that case, only one can be read at the same time.<\/li>\n<\/ul>\n\n\n\n<p>You can find in the <a href=\"https:\/\/wp.unil.ch\/fcf\/files\/2020\/09\/5L-UV-V-B-YG-R-Fluorochrome-Guide.pdf\">PDF<\/a> the list of all the signatures in order to design the panel that you want.<\/p>\n\n\n\n<p>If you are interested, please send an email to the FCF staff for more information.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The Cytek Aurora 3 is a 5 lasers spectral cytometers. Spectral Flow Cytometry provides an alternative way to measure fluorescence emission. Contrary to conventional flow cytometry which measures a limited &hellip; <\/p>\n","protected":false},"author":1001493,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_robots_primary_cat":"","_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","footnotes":""},"class_list":["post-1793","page","type-page","status-publish"],"_links":{"self":[{"href":"https:\/\/wp.unil.ch\/fcf\/wp-json\/wp\/v2\/pages\/1793","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wp.unil.ch\/fcf\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wp.unil.ch\/fcf\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wp.unil.ch\/fcf\/wp-json\/wp\/v2\/users\/1001493"}],"replies":[{"embeddable":true,"href":"https:\/\/wp.unil.ch\/fcf\/wp-json\/wp\/v2\/comments?post=1793"}],"version-history":[{"count":4,"href":"https:\/\/wp.unil.ch\/fcf\/wp-json\/wp\/v2\/pages\/1793\/revisions"}],"predecessor-version":[{"id":1859,"href":"https:\/\/wp.unil.ch\/fcf\/wp-json\/wp\/v2\/pages\/1793\/revisions\/1859"}],"wp:attachment":[{"href":"https:\/\/wp.unil.ch\/fcf\/wp-json\/wp\/v2\/media?parent=1793"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}