Prof Wurm invited Dr. Johannes F. Buyel from Aachen University to give a seminar at EPFL on Tuesday December 2nd, 2014 at 9:15 am
Dr. Buyel is a specialist of downstream processing for biopharmaceutical proteins and virus production.
The title of his seminar is:
Rational protein purification from transgenic feedstock
tobacco leaf extract as a challenging example
Abstract: Many feed streams in the biopharmaceutical production of proteins, e.g. monoclonal antibodies, have to be clarified before the dissolved target can be isolated, e.g. by chromatography. Disposable filters, flocculants and precipitation methods offer a cost-effective, fast and safe way to achieve this goal. After clarification, chromatographic unit operations are most frequently used to purify the target protein until the required purity is reached, typically >99% for pharmaceutical applications. The seminar will highlight some recent achievements in clarifying biological feedstocks derived from plants and then focus on the rational design and optimization of downstream purification strategies for recombinant target proteins. Topics such as modeling chromatographic separations and planning experimental verification will be covered.