Nuclear microsatellite (nSSR) development for diploid annual mercury

Over 3,000 microsatellite sequences have been made available for marker development by Katie Ridou, the bioinformatician responsible of Mercurialis genome assembly. These sequences include only microsatellite regions with enough flanking sequence to design 20-bp PCR primers. Jonathan El Assad is using standard methods to PCR-test several primer pairs in a diverse panel of diploid Mercurialis annua covering its full range. The target is to develop a set of about 20 microsatellites that could be used in 2-3 multiplexes. This tool would be very useful to increase our understanding of Mercurialis annua demographic history and to evaluate the levels of inbreeding in current experimental evolution and quantitative genetics experiments.