Blood Chemistry

Introduction

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The Cobas C111 robot (picture courtesy of Roche Diagnostics) is a laboratory diagnostic tool that allows routine determination of clinical chemical blood parameters, plasma enzymatic activities, specific substrates and electrolytes in blood samples of mice. Assays can be performed individually, or as “profiles” for electrolytes, metabolic substrates, or “tissue profiles”. Given the obligatory “dead volume” required, the robot is best suited for multiple analyses on each sample.

Table 1. Panels of Analyses, volumes, sampling procedure and assay price (per sample).

Note : In addition to the total analysis volume (cumulated volumes for each analyte), please add a 30ul dead volume of plasma/serum. Sample remains will be available to users after completion of the analyses.

Experimental Planning

Please contact us at least 7 days in advance to discuss the blood sampling procedure and our availability to perform the analyses.

Sample preparation should be performed as per requirements specified in Table 1.

  • Nature of the samples (to be discussed with us in advance)
    Serum
    . Collect blood into tubes (with/out coagulation activators). Incubate at room temperature for 15 minutes (clotting). Centrifuge 10’, 4,500 rpm. Transfer supernatant into Eppendorf tubes of 0.5ml preferentially (no strips), or 1.5ml. Store according to Table 1.
    Plasma. Collect blood into tubes with the appropriate anticoagulant at room temperature. Centrifuge 10’, 4,500 rpm. Transfer supernatant into Eppendorf tubes of 0.5ml preferentially (no strips), or 1.5ml. Store according to Table 1.
  • Sample volume must include analyses plus the dead volume (refer to Table 1). Please contact us for any question.
  • Sample labeling
    Tubes should be clearly labeled with easy codes (e.g. numbers #1-100) using permanent markers. Please include a list with detailed information about the nature and the number of samples.
  • Strictly avoid hemolysis
    Hemolysis can have a moderate to huge impact on the outcome of some specific tests listed in Table 1. Ways to avoid it include:

    • Never shake or whip blood samples (inversion is ok)
    • Never draw/eject too quickly blood from a capillary, needle, tip or syringe
    • During tail bleeding, do not apply too much pressure on the tail.

 

Analysis request

Please contact Gilles.Willemin@unil.ch before collecting samples . After discussion, please fill in the Analysis request form and deliver the form along with the samples.