Protocols for e-tierversuche

Protocols to be used in the Animal Experimentation Authorization Requests (e-tierversuche) will be provided on request.

Please help us updating the protocols by providing feedback on the evaluation of your authorization requests by the Canton Veterinary office (SCAV).

 

Energy Homeostasis

  • Body Composition by EchoMRI
  • Food intake
  • Indirect Calorimetry
  • Body temperature
  • Body Temperature & Locomotor Activity by telemetry
  • Metabolic response to exercise

 

Glucose Homeostasis

  • Glucose Tolerance test (oral or intraperitoneal)
  • Glucose-Stimulated Insulin Secretion in vivo
  • Insulin Tolerance test
  • Pyruvate Tolerance test
  • Bleeds in the fed-fasted-refed states
  • Euglycemic, hyperinsulinemic clamp with tracers

 

Blood Collection

  • Submandibular vein puncture
  • Saphenous vein puncture
  • Tail vein puncture

 

Urine/feces collection

  • Metabolic cages