Check out our new paper in PLoS Biology, in which we create a first functional Cdc42 GFP-tagged allele. A fast-folding variant of GFP, or mCherry, is inserted in a poorly conserved internal loop, yielding a functional sandwich fusion. We then use this allele to study the localization and dynamics of Cdc42. One of several findings is that Cdc42-GTP is significantly less mobile than Cdc42-GDP, leading to its accumulation at sites of activity.
- New paper on cell-cell fusion in the Journal of Cell Biology!
- New paper on gradient robustness